Magnesium content of mononuclear blood cells.
Magnesium content of mononuclear blood cells.
复制标题
单核血细胞的镁含量。
DOI：
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发表时间：
1985
影响因子：
9.3
通讯作者：
Jeanette M. Hosseini
中科院分区：
文献类型：
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作者：
Ronald J. Elm;Jeanette M. Hosseini
单核血细胞的镁含量可以提供比血浆或红细胞中的镁测量更好的细胞内镁或全身镁状态的评估。我们描述了一种方法来确定这一点，并报告结果为20名正常志愿者。用不连续Ficoll-Hypaque梯度分离单核细胞，洗涤，以400 X g离心，并在10 mmol/L NaCl中超声裂解。用原子吸收光谱法测定每升加入2.93克氧化镧的细胞裂解物中的镁。我们的志愿者的平均单核细胞镁含量为70.7（SD 14.1）fg/细胞和9.29（SD 1.85）mg/g DNA。镁、DNA和细胞计数测定的CV分别为3.0%、5.0%和8.7%。两种方法测定单核细胞镁含量的结果之间存在显著相关性（r = 0.67，p <0.001）。然而，通过任一方法，单核细胞、血浆或红细胞的镁浓度结果之间均无显著相关性。
The magnesium content of mononuclear blood cells may provide a better assessment of intracellular magnesium or total body magnesium status than does measurement of magnesium in plasma or erythrocytes. We describe a method for determining this, and report results for 20 normal volunteers. The mononuclear cells are separated with a discontinuous Ficoll-Hypaque gradient, washed, centrifuged at 400 X g, and lysed by sonication in 10 mmol/L NaCl. The magnesium in the cell lysate, with 2.93 g of added lanthanum oxide per liter, is determined by atomic absorption spectrometry. The mean mononuclear cell magnesium content in our volunteers was 70.7 (SD 14.1) fg per cell and 9.29 (SD 1.85) mg/g of DNA. The CVs for the determinations of magnesium, DNA, and cell count were 3.0%, 5.0%, and 8.7%, respectively. There was a significant correlation (r = 0.67, p less than 0.001) between results by the two methods of expressing magnesium content of mononuclear cells. However, by either method there were no significant correlations among results for magnesium concentration of mononuclear cells, plasma, or erythrocytes.