Cellular heat acclimation regulates cell growth, cell morphology, mitogen-activated protein kinase activation, and expression of aquaporins in mouse fibroblast cells
Cellular heat acclimation regulates cell growth, cell morphology, mitogen-activated protein kinase activation, and expression of aquaporins in mouse fibroblast cells
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细胞热驯化调节小鼠成纤维细胞中的细胞生长、细胞形态、丝裂原激活蛋白激酶激活和水通道蛋白的表达
DOI：
10.1159/000339038
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发表时间：
2012
期刊：
影响因子：
--
通讯作者：
Yachie A and Umehara H
中科院分区：
文献类型：
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作者：
Sugimoto N;Shido O;Matsuzaki K;Ohno-Shosaku T;Hitomi Y;Tanaka M;Sawaki T;Fujita Y;Kawanami T;Masaki Y;Okazaki T;Nakamura H;Koizumi S;Yachie A and Umehara H
许多研究人员对热休克反应进行了广泛的研究，以了解细胞对严重热应激(高于42°C)反应的分子机制。但是，在生理活动期间，身体或组织的温度只会上升几摄氏度。因此，细胞对轻度热应激的生理反应可能比对重度热应激更重要。热习服是人和动物的适应性生理过程，连续高温连续暴露5天可诱导热习服。然而，到目前为止，持续暴露于热应激对细胞的影响还没有得到充分的评估。在这项研究中，我们研究了在培养细胞中通过持续暴露于温和的热应激5天而诱导的适应性生理过程。暴露于热激活的p38丝裂原活化蛋白激酶；抑制细胞生长而不发生细胞凋亡；并增加小鼠成纤维细胞中HSPs和HSF-1的水平。有趣的是，热暴露调节了水通道蛋白的表达，并诱导了形态变化。在生理意义上，这些结果表明，在人和动物获得热习服的情况下，连续暴露于温和的热应激5天，可能会诱导细胞对热的分子适应。
The heat shock response has been extensively studied by a number of investigators to understand the molecular mechanism underlying the cellular response to severe heat stress (higher than 42°C). But, body or tissue temperature increases by only a few degrees Celsius during physiological events. Therefore, the physiological cellular response to mild heat stress rather than severe heat stress is likely to be more important. Repeated exposure to hyperthermia for consecutive 5 days induces heat acclimation which is an adaptive physiological process in humans and animals. However, thus far, the effect of continuous exposure to heat stress on cells has not been fully evaluated. In this study, we investigated an adaptive physiological process that is induced in culture cells by continuous exposure to mild heat stress for 5 days. Exposure to heat activated p38-mitogen-activated protein kinase; inhibited cell growth without apoptosis; and increased the levels of HSPs and HSF-1 in mouse fibroblast cells. Interestingly, exposure to heat regulated the expression of aquaporins and induced morphological change. In a physiological sense, these results suggested that continuous exposure to mild heat stress for 5 days, in which heat acclimation is attained in humans and animals, might induce molecular adaptation to heat in cells.