Expression of cytokeratin 20 in developing rat liver and in experimental models of ductular and oval cell proliferation
Expression of cytokeratin 20 in developing rat liver and in experimental models of ductular and oval cell proliferation
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DOI：
10.1016/s0168-8278(98)80158-x
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发表时间：
1998-10-01
影响因子：
25.7
通讯作者：
Desmet, VJ
中科院分区：
文献类型：
--
作者：
Faa, G;Van Eyken, P;Desmet, VJ
背景技术：最近，一种新型的细胞角蛋白（CK）作为CK 20被添加到经典的CK目录中。方法：85只Fischer大鼠分为5组：孕10 ~ 21天的胎鼠组、2 ~ 10天的新生鼠组、10 ~ 21天的正常鼠组、10 ~ 21天的正常鼠组胆管结扎（BDL）大鼠; α-萘基-异硫氰酸酯（ANIT）处理的大鼠;以及喂食含有N-2芴基乙酰胺的胆碱缺乏饮食的大鼠（CD-AAF大鼠）。用CK 20特异性单克隆抗体IT-K（S）20.10对肝脏冰冻切片进行染色。结果：CK 20为胆管型CK。在胎鼠中，CK 20在肝内胆管发育的后期出现，在妊娠第20天。出生后CK 20表达显著增加，表明出生后肝内胆管继续成熟，CK 20可被视为胆道系统的“成熟”标志物。在BDL大鼠和ANIT处理的动物中，胆管对CK 20的免疫反应性是显著异质的。不同数量的增殖胆管细胞呈CK 20弱阳性或阴性，其数量随梗阻或ANIT治疗持续时间的增加而增加。在CD-AAF治疗的大鼠中，我们发现了一个统一的染色的增殖卵圆细胞的CK 20。这一发现是在与观察BDL和ANIT组，并建议存在不同的机制，调节胆管细胞的增殖和分化，在这些conditions.Conclusions：在大鼠肝脏，CK 20可以添加到列表中的“胆管型”细胞角蛋白。在发育过程中，CK 20的表达可能与胆道系统的成熟阶段有关。在CK 20的免疫反应性方面，由BDL或ANIT喂养诱导的典型小管增殖明显不同于CD-AAF模型中的卵圆细胞增殖。
Backgound/Aiins: Recently a novel type of cytokeratin (CK) has been added to the classical catalog of CKs as CK20. The aim of the present study was to examine the immunoreactivity for CK20 in normal and developing rat liver and in experimental models of bile ductular and oval cell proliferation.Methods: Eighty-five Fischer rats, subdivided into five groups, were utilized in this study: fetal rats, ranging from day 10 to day 21 of gestation; newborn-neonatal rats, from 2 h to 10 days of age; bile duct ligated (BDL) rats; alpha-naphthyl-isothiocyanate (ANIT)-treated rats; and rats fed a choline-deficient diet containing N-2 Fluorenylacetamide (CD-AAF rats). Frozen sections from each liver were stained with the CK20 specific monoclonal antibody IT-K(S)20.10.Results: The present study shows that CK20 is a "bile duct type" CK. In the fetal rat, CK20 appears late during intrahepatic bile duct development, at day 20 of gestation. A marked increase in CK20 expression occurs after birth, suggesting that intrahepatic bile duct maturation continues after birth and that CK20 may be considered as a "maturation" marker of the biliary tree. In BDL rats and in ANIT-treated animals, immunoreactivity of bile ductules for CK20 was strikingly heterogeneous. A variable number of proliferating biliary cells were weakly positive or negative for CK20 and their number increased with the duration of the obstruction or ANIT treatment. In CD-AAF-treated rats, we found a uniform staining of proliferating oval cells for CK20. This finding is in contrast with the observation in BDL and in ANIT groups, and suggests the existence of different mechanisms regulating the proliferation and differentiation of biliary cells under those conditions.Conclusions: In rat liver, CK20 may be added to the list of "bile duct type" cytokeratins. During development, CK20 expression may be related to the maturation stage of the biliary tree. Typical ductular proliferation induced by BDL or ANIT feeding clearly differs from the oval cell proliferation in the CD-AAF model in terms of immunoreactivity for CK20.