Isometric contraction by fibroblasts and endothelial cells in tissue culture: a quantitative study.
Isometric contraction by fibroblasts and endothelial cells in tissue culture: a quantitative study.
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DOI：
10.1083/jcb.117.1.73
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发表时间：
1992-04
期刊：
影响因子：
--
通讯作者：
Wysolmerski RB
中科院分区：
文献类型：
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作者：
Kolodney MS;Wysolmerski RB
我们用等长力传感器研究了组织培养中两种非肌细胞的收缩。该方法允许定量测量由限定类型的细胞在外部试剂的影响下产生的收缩力，同时允许详细的形态学观察。鸡胚成纤维细胞（CEF），它形成一个收缩网络内的胶原蛋白基质，和人脐静脉内皮细胞（HUVE），这是位于胶原蛋白基质的表面上的单层，进行了研究。10%FCS中的CEF和HUVE分别产生4.5 +/- 0.2 × 10（4）达因/cm 2和6.1 × 10（4）达因/cm 2的显著张力。当用凝血酶刺激时，两种细胞类型都收缩，产生约10（5）达因/cm 2的每细胞横截面积的力，该值比平滑肌小约一个数量级。肌动蛋白细胞骨架的完整性对于力的产生是必不可少的，因为细胞松弛素D破坏肌动蛋白微丝会导致力的迅速消失。完整的微管似乎减少CEF施加的等长力，因为微管破坏药物导致张力增加。HUVE的收缩先于肌动蛋白微丝从周向环到应力纤维的戏剧性重排。
We have used an isometric force transducer to study contraction of two types of nonmuscle cells in tissue culture. This method permits the quantitative measurement of contractile force generated by cells of defined type under the influence of external agents while allowing detailed morphological observation. Chick embryo fibroblasts (CEF), which form a contractile network inside a collagen matrix, and human umbilical vein endothelial cells (HUVE), which are located in a monolayer on the surface of the collagen matrix, were studied. CEF and HUVE in 10% FCS produce a substantial tension of 4.5 +/- 0.2 x 10(4) dynes/cm2 and 6.1 x 10(4) dynes/cm2, respectively. Both cell types contract when stimulated with thrombin, generating a force per cell cross-sectional area of approximately 10(5) dynes/cm2, a value approximately an order of magnitude less than smooth muscle. The integrity of the actin cytoskeleton is essential for force generation, as disruption of actin microfilaments with cytochalasin D results in a rapid disappearance of force. Intact microtubules appear to reduce isometric force exerted by CEF, as microtubule-disrupting drugs result in increased tension. Contraction by HUVE precedes a dramatic rearrangement of actin microfilaments from a circumferential ring to stress fibers.