Conceptus-derived prostaglandins regulate gene expression in the endometrium prior to pregnancy recognition in ruminants.
Conceptus-derived prostaglandins regulate gene expression in the endometrium prior to pregnancy recognition in ruminants.
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DOI：
10.1530/rep-13-0165
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发表时间：
2013-10
期刊：
影响因子：
--
通讯作者：
Lonergan P
中科院分区：
文献类型：
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作者：
Spencer TE;Forde N;Dorniak P;Hansen TR;Romero JJ;Lonergan P
在牛中，囊胚在第8至9天从透明囊胚孵化，然后形成孕体，孕体在第9至16天生长并伸长成卵形，然后成丝状。生长中的孕体合成并分泌异甘草素和干扰素tau。我们的假设是，卵圆形孕体施加局部影响的子宫内膜之前，母亲承认怀孕的第16天在牛。在研究一中，同步化周期小母牛在第7天没有接受任何或接受20个体外产生的囊胚，并在第13天收集子宫。在含有多个卵圆形孕体的妊娠小母牛的子宫冲洗液中，放射免疫法未检测到干扰素tau;然而，妊娠小母牛的子宫腔内总前列腺素水平高于周期性小母牛。微阵列分析显示，44个基因增加了子宫内膜的第13天怀孕相比，周期小母牛，这些基因中的许多是经典的I型干扰素刺激的基因（ISG）。研究2和3确定了将延长的第14天孕体所产生的水平的野牡丹素输注到周期性母羊的子宫腔中对子宫内膜中ISG表达的影响。结果表明，前列腺素输注增加了子宫内膜中几种ISG的丰度。这些研究支持了这一假设，即第13天的孕体分泌的alphylandins，局部作用于旁分泌的方式改变基因表达的子宫内膜前怀孕识别牛。
In cattle, the blastocyst hatches from the zona pellucida on days 8 to 9 and then forms a conceptus that grows and elongates into an ovoid and then filamentous shape between days 9 and 16. The growing conceptus synthesizes and secretes prostaglandins and interferon tau. Our hypothesis was that the ovoid conceptus exerts a local effect on the endometrium prior to maternal recognition of pregnancy on day 16 in cattle. In Study One, synchronized cyclic heifers received nothing or 20 in vitro produced blastocysts on day 7, and uteri were collected on day 13. Interferon tau was not detected by radioimmunoassay in the uterine flush of pregnant heifers containing multiple ovoid conceptuses; however, total prostaglandin levels were higher in the uterine lumen of pregnant as compared to cyclic heifers. Microarray analysis revealed that 44 genes were increased in the endometrium of day 13 pregnant as compared to cyclic heifers, and many of those genes were classical Type I IFN-stimulated genes (ISGs). Studies Two and Three determined effects of infusing prostaglandins at the levels produced by the elongating day 14 conceptus into the uterine lumen of cyclic ewes on ISG expression in the endometrium. Results indicated that prostaglandin infusion increased the abundance of several ISGs in the endometrium. These studies support the hypothesis that the day 13 conceptus secretes prostaglandins that act locally in a paracrine manner to alter gene expression in the endometrium prior to pregnancy recognition in cattle.