The Super-Spinner: A low cost animal cell culture bioreactor for the CO2 incubator
The Super-Spinner: A low cost animal cell culture bioreactor for the CO2 incubator
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Super-Spinner：用于 CO2 培养箱的低成本动物细胞培养生物反应器
DOI：
10.1007/bf00772190
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发表时间：
2004
期刊：
影响因子：
2.2
通讯作者：
J. Lehmann
中科院分区：
文献类型：
--
作者：
R. Heidemann;U. Riese;D. Lütkemeyer;H. Büntemeyer;J. Lehmann
使用新的低成本生产系统Super Spinner进行少量单克隆抗体和重组蛋白的生产。在11标准Duran®烧瓶中安装配备有聚丙烯中空纤维膜的膜搅拌器以通过无气泡曝气改善氧气供应。通过使用CO2调节的培养箱气体促进通气，所述培养箱气体经由小型膜泵泵送通过膜搅拌器。检测了Super Spinner的最大氧传递速率（OTRmax）。为此，一个旋转烧瓶配备有氧电极。采用动态法测量OTRmax。根据所需的细胞密度，调整膜长度与培养物体积的比率，以对应于细胞的氧摄取速率。均衡的营养供应导致产品的最佳形成和产量。
The production of small quantities of monoclonal antibodies and recombinant proteins was carried out using a new low cost production system, the Super Spinner. Into a 1 1 standard Duran® flask a membrane stirrer equipped with a polypropylene hollow fiber membrane was installed to improve the oxygen supply by bubble-free aeration. The aeration was facilitated by using the CO2 conditioned incubator gas, which was pumped through the membrane stirrer via a small membrane pump. The maximal oxygen transfer rate (OTRmax) of the Super Spinner was detected. For this purpose one spinner flask was equipped with an oxygen electrode. The OTRmax was measured by the dynamic method. The ratio of membrane length to culture volume was adapted corresponding to the oxygen uptake rate of the cells according to the desired cell density. A balanced nutrient supply resulted in an optimal formation and yield of products.