Measuring Biophysical Properties of Cilia Motility from Mammalian Tissues via Quantitative Video Analysis Methods.
Measuring Biophysical Properties of Cilia Motility from Mammalian Tissues via Quantitative Video Analysis Methods.
复制标题
通过定量视频分析方法测量哺乳动物组织纤毛运动的生物物理特性。
DOI：
10.1007/978-1-0716-3507-0_16
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发表时间：
2024
期刊：
影响因子：
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通讯作者：
Causa E
中科院分区：
文献类型：
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作者：
Causa E
纤毛上皮细胞在人类的各种器官中很常见，甚至在许多物种中也很常见，它们的生理功能至关重要。许多遗传性、退行性或感染性疾病损害运动纤毛功能并导致严重的下游后果。纤毛组织培养是一种常用的研究方法。在这里，我们专注于视频显微镜和分析管道在过去几年中开发的表型纤毛跳动在肺细胞中，特别是提取：纤毛覆盖率;纤毛跳动频率分布;纤毛动态协调的规模;和纤毛跳动波形。
Ciliated epithelia are common in various human organs, indeed across many species, and their physiological functions are vital. A number of diseases, of genetic, degenerative, or infectious nature, compromise motile cilia function and lead to severe downstream consequences. Culture of ciliated tissues is a common research approach. We focus here on the video microscopy and analysis pipelines developed over the last few years to phenotype ciliary beating in lung cells, specifically to extract: cilia coverage; ciliary beat frequency distributions; the scale for ciliary dynamical coordination; and cilia beat waveform.