Succinate dehydrogenase (mitochondrial complex II) is a source of reactive oxygen species in plants and regulates development and stress responses
Succinate dehydrogenase (mitochondrial complex II) is a source of reactive oxygen species in plants and regulates development and stress responses
复制标题
DOI：
10.1111/nph.13515
复制
发表时间：
2015-11-01
期刊：
影响因子：
9.4
通讯作者：
Galina, Antonio
中科院分区：
文献类型：
--
作者：
Jardim-Messeder, Douglas;Caverzan, Andreia;Galina, Antonio
活性氧(ROS)是调节植物生长发育和胁迫反应的信号分子。线粒体是异养细胞产生ROS的主要来源，线粒体复合体I和复合体III被认为是植物线粒体产生ROS的主要部位。最近的研究表明，琥珀酸脱氢酶(SDH)也有助于线粒体ROS的产生。然而，SDH在植物中产生ROS的能力尚不清楚。本研究的目的是评估SDH在线粒体ROS产生中的作用。我们的结果表明，SDH是拟南芥和水稻ROS的直接来源，并且特定的SDH抑制剂诱导ROS的产生会损害植物的生长。此外，伴随着细胞周期基因的下调和胁迫相关基因的上调。然而，竞争性抑制剂对SDH的部分抑制减少了ROS的产生，这与增加地上部和根的生长有关，并阻止了细胞周期基因的下调和非竞争性抑制剂对胁迫相关基因的诱导。总之，SDH在ROS的产生中发挥着重要作用，SDH是植物线粒体中ROS的直接来源，调节植物的发育和胁迫响应。
Reactive oxygen species (ROS) are signaling molecules that regulate plant development and responses to stresses. Mitochondria are the source of most ROS in heterotrophic cells, and mitochondrial complex I and complex III are regarded as the main sites of ROS production in plant mitochondria. Recent studies have demonstrated that succinate dehydrogenase (SDH) also contributes to mitochondrial ROS production. However, the ability of SDH to generate ROS in plants is unclear. The aim of this study was to evaluate the role of SDH in mitochondrial ROS production.Our results demonstrated that SDH is a direct source of ROS in Arabidopsis thaliana and Oryza sativa, and the induction of ROS production by specific SDH inhibitors impaired plant growth. In addition, this effect was accompanied by the down-regulation of cell cycle genes and the up-regulation of stress-related genes.However, the partial inhibition of SDH by a competitive inhibitor decreased ROS production, which was associated with increased shoot and root growth, and prevented the down-regulation of cell cycle genes and the induction of stress-related genes by noncompetitive inhibitors.In conclusion, SDH plays an important role in ROS production, being a direct source of ROS in plant mitochondria and regulating plant development and stress responses.