Characterization of embryonic globin genes of the zebrafish
Characterization of embryonic globin genes of the zebrafish
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DOI：
10.1016/s0012-1606(02)00041-6
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发表时间：
2003-03-01
影响因子：
2.7
通讯作者：
Zon, L
中科院分区：
文献类型：
--
作者：
Brownlie, A;Hersey, C;Zon, L
血红蛋白转换是一个复杂的过程，在发育阶段产生不同的珠蛋白链。为了表征斑马鱼模型系统中血红蛋白转换的过程，我们分离并表征了几个胚胎珠蛋白基因。胚胎和成人的珠蛋白基因以头对头的结构聚集在一起。一个胚胎和成人基因簇定位在连锁组3，而另一个胚胎基因簇定位在连锁组12。几个胚胎珠蛋白基因在胚胎发生早期表现出红细胞特异性的表达模式，后来随着最终造血发生而下调。我们利用电喷雾质谱分析了发育中的胚胎红细胞中球蛋白基因和蛋白表达的相关性。突变的仙粉黛（zinfandel）在胚胎时期患有低色素小细胞性贫血，但后来在成年后恢复。仙粉黛基因定位在靠近主要珠蛋白基因位点的连锁组3，强烈提示仙粉黛代表胚胎珠蛋白缺陷。我们的研究是第一个系统地评估斑马鱼胚胎球蛋白的研究，最终将有助于评估斑马鱼血红蛋白产生和转换缺陷的突变体。(C) 2003 Elsevier Science（美国）版权所有。
Hemoglobin switching is a complex process by which distinct globin chains are produced during stages of development. In an effort to characterize the process of hemoglobin switching in the zebrafish model system, we have isolated and characterized several embryonic globin genes. The embryonic and adult globin genes are found in clusters in a head-to-head configuration. One cluster of embryonic and adult genes is localized to linkage group 3, whereas another embryonic cluster is localized on linkage group 12. Several embryonic globin genes demonstrate an erythroid-specific pattern of expression early during embryogenesis and later are downregulated as definitive hematopoiesis occurs. We utilized electrospray mass spectroscopy to correlate globin genes and protein expression in developing embryonic red cells. The mutation, zinfandel, has a hypochromic microcytic anemia as an embryo, but later recovers in adulthood. The zinfandel gene maps to linkage group 3 near the major globin gene locus, strongly suggesting that zinfandel represents an embryonic globin defect. Our studies are the first to systematically evaluate the embryonic globins in the zebrafish and will ultimately be useful in evaluating zebrafish mutants with defects in hemoglobin production and switching. (C) 2003 Elsevier Science (USA). All rights reserved.