The determination of specific radioactivity of proteins eluted intact from polyacrylamide gels, utilizing a fluorescamine assay.
The determination of specific radioactivity of proteins eluted intact from polyacrylamide gels, utilizing a fluorescamine assay.
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利用荧光胺测定法测定从聚丙烯酰胺凝胶中完整洗脱的蛋白质的特异性放射性。
DOI：
10.1016/0003-2697(85)90335-5
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发表时间：
1985
影响因子：
2.9
通讯作者：
Fisher,WR
中科院分区：
文献类型：
--
作者：
Kilgore,LL;Rogers,JL;Patterson,BW;Miller,NH;Fisher,WR
所描述的方法允许测量不同蛋白质的比放射性，这些蛋白质可通过使用圆柱形聚丙烯酰胺凝胶的分离技术来分辨。分离后，对蛋白质进行电洗脱;使用荧光胺测定法在微克范围内定量洗脱的蛋白质，而回收样品的大部分用于放射性测定。这些程序已适用于蛋白质代谢的示踪研究。它们在动力学研究中的效用被证明与改变的人血浆白蛋白和载脂蛋白B在体内用[3 H]亮氨酸示踪剂标记的特定放射性的时间过程中的数据。
The methodology described permits the measurement of the specific radioactivity of diverse proteins resolvable by separatory techniques using cylindrical polyacrylamide gels. Following separation, the proteins are electroeluted; eluted protein is quantitated in the microgram range using a fluorescamine assay, while the major portion of the recovered sample is used for radioactivity measurement. These procedures have been adapted for use in tracer studies of protein metabolism. Their utility in kinetic investigations is demonstrated with data on the time course of changing specific radioactivities of human plasma albumin and apolipoprotein B labeled in vivo with a [3H]leucine tracer.