LIPOPOLYSACCHARIDE BINDING-PROTEIN AND CD14 IN LPS DEPENDENT MACROPHAGE ACTIVATION
LIPOPOLYSACCHARIDE BINDING-PROTEIN AND CD14 IN LPS DEPENDENT MACROPHAGE ACTIVATION
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DOI：
10.1016/s0171-2985(11)80341-4
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发表时间：
1993-04-01
期刊：
影响因子：
2.8
通讯作者：
ULEVITCH, RJ
中科院分区：
文献类型：
--
作者：
TOBIAS, PS;ULEVITCH, RJ
脂多糖(LPS)激活单核细胞、巨噬细胞和中性粒细胞涉及一种血清蛋白，即脂多糖结合蛋白(LBP)和一种膜蛋白，即CD14。迄今为止的证据表明，血清中的内毒素首先与LBP结合。然后，内毒素-LBP复合体与CD14相互作用，导致细胞激活。这一方案得到了LBP或CD14被抗体阻断的实验以及将LBP添加到无血清介质中并在通常缺乏CD14的细胞上表达CD14的实验的支持。这一途径的发现为抗内毒素治疗提供了新的途径。
The activation of monocytes, macrophages, and neutrophils by lipopolysaccharides (LPS) involves a serum protein, LPS binding protein (LBP), and a membrane protein, CD14. Evidence to date suggests a pathway in which serum LPS first binds to LBP. The LPS-LBP complexes then interact with CD14, leading to cellular activation. This scheme is supported by experiments in which either LBP or CD14 are blocked by antibodies as well as experiments in which LBP is added to serum free media and CD14 is expressed on cells which normally lack CD14. Discovery of this pathway suggests novel approaches to anti-LPS therapy.