COMPARISON OF INVITRO ACTIVITY OF CYTOTOXIC DRUGS TOWARDS HUMAN CARCINOMA AND LEUKEMIA-CELL LINES
COMPARISON OF INVITRO ACTIVITY OF CYTOTOXIC DRUGS TOWARDS HUMAN CARCINOMA AND LEUKEMIA-CELL LINES
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DOI：
10.1016/0277-5379(86)90162-8
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发表时间：
1986-06-01
期刊：
影响因子：
--
通讯作者：
BAGULEY, BC
中科院分区：
文献类型：
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作者：
FINLAY, GJ;WILSON, WR;BAGULEY, BC
使用八种人类造血细胞系和四种人类癌细胞系来比较多种细胞毒性药物的活性，包括安吖啶、安吖啶类似物CI-921、甲氨蝶呤、硝吖啶、阿霉素、柔红霉素和5-氟尿嘧啶。通过半自动微培养物生长抑制测定来评估活性。使用Mosmann的技术测量非贴壁细胞系的细胞密度(JImmunolMethods 1983,65,55-63)，其中染料噻唑蓝(MTT)代谢成深蓝色甲臜产物。该技术给出了与在电子细胞计数器中直接细胞计数获得的结果类似的结果，并且当应用于一些贴壁细胞系时，给出了与先前开发的亚甲蓝染色技术获得的结果类似的结果（Anal Biochem 1984, 139, 272-277）。亚甲蓝和 MTT 方法均用于结合半自动 96 孔微量培养板技术研究细胞毒性。结果表明，三种 T 细胞白血病系（CCRF-CEM、Jurkat 和 MOLT-4）比结肠癌系（HCT-8、HT-29、SW480 和 SW620）对 DNA 结合药物（不包括硝克林）更敏感。耐药性更强的造血系的药物敏感性介于 T 细胞白血病和癌细胞系之间。 DNA 结合药物对不同细胞系表现出非常相似的差异活性模式。
Eight human haematopoietic cell lines and four human carcinoma lines were used to compare the activity of a number of cytotoxic drugs including ansacrine, the ansacrine analogue CI-921, methotrexate, nitracrine, doxorubicin, daunorubicin and 5-fluorouracil. Activity was assessed by means of semiautomated microculture growth inhibition assays. Cell density of the non-adherent cell lines was measured using the techinique of Mosmann (J Immunol Methods 1983, 65, 55-63), in which the dye thiazolyl blue (MTT) is metabolised to a dark blue formazan product. This technique gives similar results to those obtained by direct cell counting in an electronic cell counter, and when applied to some adherent cell lines gives similar results to those obtained by the methylene blue staining technique previously developed (Anal Biochem 1984, 139, 272-277). Both methylene blue and MTT methods were used to investigate cytotoxicity in conjunction with semi-automatead 96-well microculture plate techniques. The results show that the three T-cell leukaemia lines (CCRF-CEM, Jurkat and MOLT-4) are more sensitive to DNA-binding drugs (excluding nitracrine) than are the colon carcinoma lines (HCT-8, HT-29, SW480 and SW620). The more resistant haematopoietic lines are intermediate in drug sensitivity between the T cell leukaemia and carcinoma lines. The DNA binding drugs show remarkably similar patterns of differential activity against the different cell lines.