Structural elucidation and antidiabetic activity of fucosylated chondroitin sulfate from sea cucumber Stichopus japonicas
Structural elucidation and antidiabetic activity of fucosylated chondroitin sulfate from sea cucumber Stichopus japonicas
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DOI：
10.1016/j.carbpol.2021.117969
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发表时间：
2021-03-24
影响因子：
11.2
通讯作者：
Li, Hui-Jing
中科院分区：
文献类型：
--
作者：
Gong, Pi-Xian;Li, Qin-Ying;Li, Hui-Jing
从海参Stichopus japonicus （FCSsj）体壁中分离到一种硫酸软骨素，并用核磁共振波谱和HILIC-FTMS对其结构进行了表征。在1.00:0.26:0.65的比例下，发现了3个聚焦基残基：2,4-二硫化-聚焦（Fuc2,4S）、4-硫酸化-聚焦（Fuc4S）和3,4-二硫化-聚焦（Fuc3,4S），它们仅与葡萄糖醛酸残基（GlcA）的O-3相连。除了单聚焦基外，双聚焦基分支，即Fuc2,4S α (1 -> 3)Fuc4S，也被发现附着在GlcA的O-3上。采用体外葡萄糖胺诱导胰岛素抵抗（IR） Hep G2细胞，评价了fcsj的抗糖尿病活性。结果发现，fcsj显著促进IR-Hep G2细胞的葡萄糖摄取和葡萄糖消耗，并呈剂量依赖性，可减轻细胞损伤。此外，fcsj还能促进葡萄糖胺诱导的IR-Hep G2细胞糖原的合成。这些结果为研究fcsj的抗糖尿病活性提供了补充。
A fucosylated chondroitin sulfate was isolated from the body wall of sea cucumber Stichopus japonicus (FCSsj), whose structure was characterized by NMR spectroscopy and HILIC-FTMS. At the ratio of 1.00:0.26:0.65, three fucosyl residues were found: 2,4-disulfated-fucose (Fuc2,4S), 4-sulfated-fucose (Fuc4S) and 3,4-disulfated-fucose (Fuc3,4S), which were only linked to the O-3 of glucuronic acid residues (GlcA). Besides mono-fucosyl moieties, di-fucosyl branches, namely Fuc2,4S alpha(1 -> 3)Fuc4S, were also found to be attached to the O-3 of GlcA. The antidiabetic activity of FCSsj was evaluated using glucosamine induced insulin resistant (IR) Hep G2 cells in vitro. It was found that FCSsj significantly promoted the glucose uptake and glucose consumption of IR-Hep G2 cells in a dose-dependent manner, and could alleviate the cell damage. Furthermore, FCSsj could promote the glycogen synthesis in the glucosamine-induced IR-Hep G2 cells. These results provided a supplement for studying the antidiabetic activity of FCSsj.