The homeo domain of a murine protein binds 5' to its own homeo box.
The homeo domain of a murine protein binds 5' to its own homeo box.
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鼠类蛋白的同源结构域与其自身的同源框结合 5。
DOI：
10.1073/pnas.83.24.9532
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发表时间：
1986
影响因子：
11.1
通讯作者：
Ruddle,FH
中科院分区：
文献类型：
--
作者：
Fainsod,A;Bogarad,LD;Ruusala,T;Lubin,M;Crothers,DM;Ruddle,FH
来自12.5天小鼠胚胎的核蛋白提取物用于研究蛋白质与Hox 1.5同源盒的DNA序列5'的结合。已知这个发育阶段的胚胎表达该基因。DNA结合蛋白印迹和阻滞凝胶技术表明，小鼠胚胎核蛋白特异性结合753碱基对（bp）的DNA片段从上游区域的Hox 1.5同源盒。在λ gt 11中构建的含有Hox 1.5同源结构域的融合蛋白也结合相同的753-bp DNA片段。融合蛋白与上游DNA片段的特异性结合表明，同源盒含有特异性蛋白质-DNA相互作用所需的序列，并且753-bp片段含有同源结构域结合位点。这些结果支持小鼠同源盒是参与胚胎发育调控的蛋白质的DNA结合域的假设。
Nuclear protein extracts from day 12.5 mouse embryos were used to study protein binding to DNA sequences 5' of the Hox 1.5 homeo box. Embryos of this developmental stage are known to express this gene. DNA binding protein blotting and retardation gel techniques show that murine embryonic nuclear proteins specifically bind a 753-base pair (bp) DNA fragment from the region upstream of the Hox 1.5 homeo box. A fusion protein containing the Hox 1.5 homeo domain constructed in lambda gt11 also binds the same 753-bp DNA fragment. Specific binding of the fusion protein to the upstream DNA fragment shows that the homeo box contains the sequences required for specific protein-DNA interactions, and the 753-bp fragment contains a homeo domain binding site. These results support the hypothesis that murine homeo boxes are DNA binding domains of proteins involved in the regulation of embryonic development.