Genetic diversity within grouper species and a method for interspecific hybrid identification using DNA barcoding and RYR3 marker.
Genetic diversity within grouper species and a method for interspecific hybrid identification using DNA barcoding and RYR3 marker.
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DOI：
10.1016/j.ympev.2017.12.031
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发表时间：
2017-12
影响因子：
4.1
通讯作者：
M. Qu;Wei Tang;Qiaohong Liu;Dexiang Wang;S. Ding
中科院分区：
文献类型：
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作者：
M. Qu;Wei Tang;Qiaohong Liu;Dexiang Wang;S. Ding
石斑鱼（石斑鱼科）是珊瑚礁鱼类的一个组合，包括16属160多个物种，其中许多都是环境和经济价值。由于其相似的形态，多变的颜色模式，并倾向于种间杂交，形态鉴定石斑鱼通常会导致分类混乱。为了寻找一种有效的方法来鉴定不同种类的石斑鱼和杂交种，评估遗传多样性和发现任何同义或隐藏的物种，我们在中国海域共采集了221个标本，代表9属57种。线粒体（mt）细胞色素氧化酶亚基I（COI）和NADH脱氢酶亚基2（ND 2）被发现是有效的条形码基因。我们还开发了一个有效的协议，用于识别杂交石斑鱼使用mt标记和核RYR3基因，并发现了广泛的种间杂交的第一个记录在属Epinealus。这项条形码研究揭示了许多广泛分布的物种和可能的同义词的高度遗传差异。本研究除了为石斑鱼物种鉴定提供分子方法外，还为石斑鱼保护遗传学、物种形成、杂交等进化性状的进一步研究提供了重要资源。
Groupers (family Epinephelidae) are an assemblage of coral reef fishes comprising more than 160 species in 16 genera, many of which are both environmentally and economically valuable. Because of their similar morphology, variable color patterns, and tendency for interspecies hybridization, morphological identification of groupers usually leads to taxonomic confusion. To find an effective method for identifying different grouper species and hybrids, evaluate genetic diversity and uncover any synonymous or cryptic species, we sampled a total of 221 specimens representing 57 species in 9 genera in the China Seas. Both mitochondrial (mt) cytochrome oxidase subunit I (COI) and NADH dehydrogenase subunit 2 (ND2) were found to be effective barcoding genes. We also developed an efficient protocol for identifying hybrid groupers using mt markers and the nuclear RYR3 gene and found the first record of wide interspecies hybridization in genusEpinephelus. This barcoding study revealed high genetic divergence in many widespread species and possible synonyms. In addition to providing a molecular method for identifying grouper species, this study offers important resources for the further study of grouper conservation genetics, speciation, hybridization and other evolutionary traits.