ANALYSIS OF CELL RECOMBINATIONS IN EXPERIMENTAL SYNTHESIS OF TISSUES IN VITRO
ANALYSIS OF CELL RECOMBINATIONS IN EXPERIMENTAL SYNTHESIS OF TISSUES IN VITRO
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DOI：
10.1002/jcp.1030600407
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发表时间：
1962-01-01
影响因子：
--
通讯作者：
MOSCONA, AA
中科院分区：
文献类型：
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作者：
MOSCONA, AA
本文描述了：（1）鸡和小鼠胚胎离散细胞的体外组织型聚集，重点是种内和种间细胞组合中的选择性接触和分组现象；（2）有关离散细胞组织发生性凝聚机制的近期实验。旋转聚集的方法提供了一个易于控制的实验系统，适用于对离散细胞的组织发生相互作用进行定量研究。研究了环境（低温、高碘酸盐）和细胞（年龄、单层生长）参数对细胞凝聚能力和组织型联合的限制作用。获得了无细胞上清液，其在次优温度下显著促进了离散细胞的再凝聚。对这些上清液的细胞类型特异性进行了检测。所提供的证据表明，上清液的特征性细胞结合活性取决于细胞释放的产物。这些结果证实了先前提出的工作概念，即胚胎细胞的组织型再联合可能涉及特定细胞外或细胞表面物质的功能。从近期发现以及其他系统中的类似情况的角度对这一假设进行了重新审视。
The paper describes: (1) histotypic aggregation in vitro of dissociated cells of chick and mouse embryos, with emphasis on selective contact and grouping phenomena in intra-and interspecific cell combinations and (2) recent experiments bearing on the mechanism of histogenetic cohesion of dissociated cells. The procedure of aggregation by rotation provides a readily controllable experimental system suitable for quantitative studies on histogenetic interactions of dissociated cells. The restrictive effects of environmental (low temperatures, periodate) and cellular (age, growth in mono-layers) parameters on cohesive competence and histotypic association of cells were studied. Cell-free supernatants were obtained which promoted markedly the recohesion of dissociated cells at suboptimal temperatures. The cell-type specificity of these supernatants was examined. Evidence presented indicates that the characteristic cell-binding activity of the supernatants depends on products released by the cells. These results corroborate the previously advanced working concept that histotypic reassociation of embryonic cells may involve the function of specific extracellular or cell-surface materials. This assumption is re-examined in the perspective of recent findings and of analogous situations in other systems.