A de novo transcriptome assembly approach elucidates the dynamics of ovarian maturation in the swordfish (Xiphias gladius)
A de novo transcriptome assembly approach elucidates the dynamics of ovarian maturation in the swordfish (Xiphias gladius)
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DOI：
10.1038/s41598-019-43872-6
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发表时间：
2019-05-14
影响因子：
4.6
通讯作者：
Carnevali, Oliana
中科院分区：
文献类型：
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作者：
Gioacchini, Giorgia;Marisaldi, Luca;Carnevali, Oliana
地中海箭鱼（Xiphias gladius）最近被列为过度捕捞，2016年，国际大西洋金枪鱼保护委员会（ICCAT）制定了一项多年度管理计划，以恢复这一种群。为了成功地实现这一目标，需要了解旗鱼生物学。迄今为止，对箭鱼的研究很少，没有一个在分子水平上提供有用的见解生殖生物学。在这里，我们设置到卵巢成熟的分子动力学基础上采用从头转录组组装的方法。成熟和未成熟卵巢的差异基因表达分析鉴定了许多与驱动卵巢成熟的生物学过程相关的差异表达基因。专注于卵巢类固醇和卵黄蛋白原摄取，我们描绘了这些过程的分子动力学特征，而系统发育分析让我们确定一个候选人卵黄蛋白原受体。这是第一个旗鱼转录组组装，这些发现提供了深入了解描述卵巢成熟的分子过程。此外，建立一个载有箭鱼转录组信息的公开数据库的目的是促进对这一物种的研究，以便制定更全面和更成功的种群管理计划。
The Mediterranean swordfish (Xiphias gladius) has been recently classified as overfished and in 2016, the International Commission for the Conservation of the Atlantic Tunas (ICCAT) established a multi-annual management plan to recover this stock. To successfully achieve this goal, knowledge about swordfish biology is needed. To date, few studies on swordfish have been performed and none of them has provided useful insights into the reproductive biology at molecular level. Here we set to characterise the molecular dynamics underlying ovarian maturation by employing a de novo transcriptome assembly approach. Differential gene expression analysis in mature and immature ovaries identified a number of differentially expressed genes associated with biological processes driving ovarian maturation. Focusing on ovarian steroidogenesis and vitellogenin uptake, we depict the molecular dynamics characterizing these processes while a phylogenetic analysis let us identify a candidate vitellogenin receptor. This is the first swordfish transcriptome assembly and these findings provide in-depth understanding of molecular processes describing ovarian maturation. Moreover, the establishment of a publicly available database containing information on the swordfish transcriptome aims to boost research on this species with the long-term of developing more comprehensive and successful stock management plans.