Myoblasts Differentiated From Adipose-derived Stem Cells to Treat Stress Urinary Incontinence
Myoblasts Differentiated From Adipose-derived Stem Cells to Treat Stress Urinary Incontinence
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DOI：
10.1016/j.urology.2009.10.003
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发表时间：
2010-03-01
期刊：
影响因子：
2.1
通讯作者：
Cui, Lei
中科院分区：
文献类型：
--
作者：
Fu, Qiang;Song, Xiao-Fei;Cui, Lei
目的探讨脂肪干细胞（ADSC）技术在压力性尿失禁治疗中的应用。方法采用阴道球囊扩张法建立压力性尿失禁动物模型（20只雌性Sprague-Dawley大鼠），并进行尿动力学和组织学检查。收集内源性大鼠ADSCs，采用5-Aza诱导技术在体外诱导成肌细胞。通过结蛋白和肌球蛋白的免疫荧光标记证实了成肌细胞的身份。将诱导细胞注射到压力性尿失禁动物膀胱颈的后尿道肌层中。 1 个月和 3 个月后通过尿动力学和组织学检查效果。还植入未经处理的 ADSC 作为对照方法。 结果 与对照相比，植入后 1 个月和 3 个月后最大膀胱容量和漏点压力均显着增加 (P < .05)。观察到尿道粘膜下肌层厚度增加，大纵向肌束数量增多。 α-平滑肌动蛋白的免疫化学分析表明，粘膜下出现的成肌细胞数量增加。结论 ADSC 具有分化成多种谱系的能力，包括成肌细胞。这种诱导成肌细胞的能力可用于治疗压力性尿失禁，具有侵入最小、恢复较快的优点。泌尿学 75: 718-723, 2010。(C) 2010 Elsevier Inc.
OBJECTIVES To investigate the application of adipose-derived stem cell (ADSC) technology in the treatment of stress incontinence.METHODS The vaginal balloon dilatation method was used to establish an animal model of stress incontinence (in 20 female Sprague-Dawley rats), which was further examined by urodynamics and histology. Endogenous rat ADSCs were collected and induced into myoblasts with 5-Aza induction technology in vitro. The identity of myoblasts was confirmed through immunofluorescence labeling with desmin and myosin. Induced cells were injected into the posterior urethral muscularis in the bladder neck of animals with stress incontinence. The effects were examined after 1 and 3 months by urodynamics and histology. Untreated ADSCs were also implanted as a method of control.RESULTS Both maximal bladder capacity and leak point pressure significantly increased after 1 and 3 months postimplantation, compared with the control (P < .05). Increased thickness of inferior muscularis in urethral mucosa and a greater number of large longitudinal muscle bundles were observed. Increased numbers of myoblasts appeared under the mucosa, as demonstrated by the immunochemistry analysis of alpha-smooth actin.CONCLUSIONS ADSCs have the ability of differentiating into multiple lineages, including myoblasts. This ability to induce myoblasts can be used to treat stress incontinence, with the advantages of minimal invasion and faster recovery. UROLOGY 75: 718-723, 2010. (C) 2010 Elsevier Inc.