The retinoid X receptor from mud crab: new insights into its roles in ovarian development and related signaling pathway.
The retinoid X receptor from mud crab: new insights into its roles in ovarian development and related signaling pathway.
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泥蟹类视黄醇X受体：对其在卵巢发育和相关信号通路中的作用的新见解
DOI：
10.1038/srep23654
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发表时间：
2016-03-24
影响因子：
4.6
通讯作者：
Li S
中科院分区：
文献类型：
--
作者：
Gong J;Huang C;Shu L;Bao C;Huang H;Ye H;Zeng C;Li S
在节肢动物中，类维生素A X受体（RXR）是一种高度保守的核激素受体。通过与蜕皮激素受体（EcR）形成异二聚体复合物，已知RXR对各种生理过程至关重要。然而，与EcR相比，RXR信号通路及其在甲壳动物繁殖中的作用知之甚少。本研究在拟穴青蟹（Scylla paramamosain，SpRXR）卵巢滤泡细胞中检测到RXR mRNA的表达，在卵巢成熟过程中，其表达量显著增加，体外实验表明，20-羟基蜕皮激素（20-hydroxyecdysone，20 E）能显著诱导SpRXR和卵黄蛋白原（vitellogenin，SpVg）mRNA的表达，而法呢酸甲酯（methyl farnesoate，MF）对SpRXR和SpVg mRNA的表达无明显影响。但与体外实验不同的是，体内实验中注射MF可显著刺激卵黄发生早期雌蟹SpRXR和SpVg的表达，但不诱导蜕皮激素和昆虫保幼激素（JH）信号通路基因的表达。以上结果提示MF和SpRXR在调节SpVg表达和卵巢发育中起重要作用。拟穴蛙通过自己的特定信号通路而不是与蜕皮激素或昆虫JH共享。
In arthropods, retinoid X receptor (RXR) is a highly conserved nuclear hormone receptor. By forming a heterodimeric complex with the ecdysone receptor (EcR), RXR is known to be vital importance for various physiological processes. However, in comparison to EcR, the RXR signaling pathway and its roles in crustacean reproduction are poorly understood. In the present study, the RXR mRNA was detected in the ovarian follicular cells of mud crabScylla paramamosain(SpRXR) and during ovarian maturation, its expression level was found to increase significantly.In vitroexperiment showed that both SpRXR and vitellogenin (SpVg) mRNA in the ovarian explants were significantly induced by 20-hydroxyecdysone (20E) but not methyl farnesoate (MF). However, differing from thein vitroexperiment, injection of MF inin vivoexperiment significantly stimulated the expressions of SpRXR and SpVg in female crabs at early vitellogenic stage, but the ecdysone and insect juvenile hormone (JH) signaling pathway genes were not induced. The results together suggest that both MF and SpRXR play significant roles in regulating the expression of SpVg and ovarian development ofS. paramamosainthrough their own specific signaling pathway rather than sharing with the ecdysone or the insect JH.