Solubilization of enzymes in water-in-oil microemulsions and their rapid and efficient release through use of a pH-degradable surfactant.
Solubilization of enzymes in water-in-oil microemulsions and their rapid and efficient release through use of a pH-degradable surfactant.
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通过使用 pH 可降解的表面活性剂，将酶溶解在油包水微乳液中并快速有效地释放。
DOI：
10.1007/s10529-006-9292-3
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发表时间：
2007
影响因子：
2.7
通讯作者：
Harris,JMilton
中科院分区：
文献类型：
--
作者：
Rairkar,MaithiliE;Hayes,DouglasG;Harris,JMilton
α-胰凝乳蛋白酶和溶菌酶分别以1.5-2和10 g l− 1的浓度溶解于水/O-[（2-十三烷基，2-乙基-1，3-二氧戊环-4-基）甲氧基]-O′-甲氧基聚乙二醇（CK-2，1,3表面活性剂）/异辛烷油包水微乳液溶液中，得到0.15和1.2MCK-2，1,3。当与等体积的0.1MNaH_2PO_4/Na_2HPO_4缓冲液（pH 5）接触时，形成由富含表面活性剂的中间相、水相和富含异辛烷的“过量”相组成的三相体系（Winsor-III体系）。这两种酶被迅速释放到水过量相，与70%的回收率在30和60分钟的微乳液溶液中含有0.15和1.2M的表面活性剂，分别。回收的酶保留了>90%的原始比活性。
α-Chymotrypsin and lysozyme were solubilized in a water/O-[(2-tridecyl, 2-ethyl-1,3-dioxolan-4-yl)methoxy]–O′-methoxy poly(ethylene glycol) (CK-2,13 surfactant)/isooctane water-in-oil microemulsion solution at 1.5–2 and 10 g l−1for 0.15 and 1.2MCK-2,13, respectively. Upon contact with an equal volume of 0.1MNaH2PO4/Na2HPO4buffer, pH 5, a three-phase system (Winsor-III system) was formed, consisting of a surfactant-rich middle phase and aqueous and isooctane-rich “excess” phases. Both enzymes were rapidly released into the aqueous excess phase, with 70% recovery of each in 30 and 60 min for microemulsion solutions containing 0.15 and 1.2Msurfactant, respectively. The recovered enzymes retained >90% of their original specific activity.