E-Cadherin and α-Catenin Expression in Human Esophageal Cancer
E-Cadherin and α-Catenin Expression in Human Esophageal Cancer
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发表时间：
1994
期刊：
影响因子：
11.2
通讯作者：
T. Kadowaki;H. Shiozaki;M. Inoue;S. Tamura;H. Oka;Y. Doki;K. Iihara;S. Matsui;T. Iwazawa;A. Nagafuchi;S. Tsukita;T. Mori
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作者：
T. Kadowaki;H. Shiozaki;M. Inoue;S. Tamura;H. Oka;Y. Doki;K. Iihara;S. Matsui;T. Iwazawa;A. Nagafuchi;S. Tsukita;T. Mori
上皮组织的细胞间粘附主要受E-钙粘蛋白（E-cad）分子的调节。α-Catenin（α-cat）是一种与E-cad相关的胞质蛋白，它与细胞骨架形成连接并调节E-cad功能。为探讨食管癌组织中细胞间粘附功能障碍的机制，我们采用免疫组化方法检测了46例食管癌组织中E-cad和α-cat的表达。通过将E-cad和α-cat表达分级为均匀阳性（+）、异质性（±）或均匀阴性（−），46例肿瘤可分为9例（20%）E-cad（+）/α-cat（+）、15例（33%）E-cad（±）/α-cat（±）、21例（46%）E-cad（±）/α-cat（−）和1例（2%）E-cad（−）/α-cat（−）。46个肿瘤中的25个（54%）显示出两种分子的相似表达，而其他21个肿瘤（46%）显示出E-cad（±）/α-cat（−）。因此，尽管α-cat的表达与E-cad的表达显着相关，但在某些肿瘤中α-cat的减少幅度更大。在临床病理特征方面，α-cat和E-cad的表达降低与肿瘤的去分化、浸润性生长和淋巴结转移显著相关（P <0.05）。这些结果提示，E-cad和α-cat都是细胞间粘附的重要调节因子，α-cat也参与了肿瘤的侵袭和转移。特别是，α-cat表达的降低与人食管癌的浸润表型和淋巴结转移的相关性比E-cad表达的相关性更高。这篇文章的出版费用部分由版面费支付。因此，本文必须根据18 U.S.C.在此标记为广告。第1734条仅仅是为了表明这一事实。
Abstract Intercellular adhesion of the epithelial tissue is mainly regulated by the E-cadherin (E-cad) molecule. α-Catenin (α-cat) is one of the E-cad-associated cytoplasmic proteins that forms a linkage to the cytoskeleton and regulates E-cad function. To investigate the mechanism of dysfunction in cell-cell adhesion in cancerous tissues, we examined E-cad and α-cat expression by immunohistochemical staining on 46 human esophageal cancers using our specific monoclonal antibodies. By grading of E-cad and α-cat expression as uniformly positive (+), heterogeneous (±), or uniformly negative (−), the 46 tumors could be classified into 9 (20%) E-cad(+)/α-cat(+), 15 (33%) E-cad(±)/α-cat(±), 21 (46%) E-cad(±)/α-cat(−), and 1 (2%) E-cad(−)/α-cat(−). Twenty-five (54%) of the 46 tumors showed a similar expression of both molecules, while the other 21 tumors (46%) showed E-cad(±)/α-cat(−). Thus, although the expression of α-cat was significantly correlated with that of E-cad, in some tumors the reduction of α-cat was greater. Regarding the clinicopathological features, the reduction of α-cat expression, as well as that of E-cad, was significantly associated with tumor dedifferentiation, infiltrative growth, and lymph node metastasis (P These results suggest that not only E-cad but also α-cat are important regulators of intercellular adhesion and that α-cat is also involved in invasion and metastasis. In particular, reduction of α-cat expression is more correlated with invasive phenotype and lymph node metastasis than E-cad expression in human esophageal cancer. The costs of publication of this article were defrayed in part by the payment of page charges. This article must therefore be hereby marked advertisement in accordance with 18 U.S.C. Section 1734 solely to indicate this fact.