PKR - A protein kinase regulated by double-stranded RNA
PKR - A protein kinase regulated by double-stranded RNA
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DOI：
10.1016/s1357-2725(96)00169-0
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发表时间：
1997-07-01
影响因子：
4
通讯作者：
Clemens, MJ
中科院分区：
文献类型：
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作者：
Clemens, MJ
RNA调节蛋白激酶（PKR）是一种广泛存在于真核生物中的干扰素诱导酶。这种丝氨酸/苏氨酸特异性蛋白激酶通过涉及自磷酸化的机制被双链RNA激活。一旦被激活，该酶就会磷酸化a！最近证据表明，可能存在另外底物，信号转导和基因转录途径也可能受蛋白激酶的调节。除了在介导干扰素的抗病毒作用中是重要的之外，PKR还涉及调节未感染细胞中的细胞增殖，并且在正常条件下可能具有肿瘤抑制功能。使用表达PKR的失活突变体的细胞系和具有PKR基因的纯合破坏的小鼠的研究导致对该酶的生物学意义的更深入的了解，（C）1997 Elsevier Science Ltd.
The RNA-regulated protein kinase, (PKR) is an interferon-iuducible enzyme of widespread occurrence in eukaryotic organisms. This serine/threonine-specific protein kinase is activated by double-stranded RNA by a mechanism involving autophosphorylation. Once activated, the enzyme phosphorylates the a! subunit of protein synthesis initiation factor eIF2, thereby inhibiting translation, Recent evidence suggests that there may be additional substrates, and that signal transduction and gene transcription path ways also may be regulated by the protein kinase. As well as being important in mediating the antiviral effects of interferons, PKR is implicated in regulating cell proliferation in uninfected cells and may have a tumour suppressor function under normal conditions, Studies using cell lines expressing inactive mutants of PKR and mice with homozygous disruptions of the PKR gene are leading to greater insights into the biological significance of this enzyme, (C) 1997 Elsevier Science Ltd.