A tektin homologue is decreased in Chlamydomonas mutants lacking an axonemal inner-arm dynein
A tektin homologue is decreased in Chlamydomonas mutants lacking an axonemal inner-arm dynein
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DOI：
10.1091/mbc.e03-11-0854
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发表时间：
2004-05-01
影响因子：
3.3
通讯作者：
Kamiya, R
中科院分区：
文献类型：
--
作者：
Yanagisawa, H;Kamiya, R
在纤毛和鞭毛轴丝中，各种离散的结构，如内部和外部动力蛋白臂规则地排列在外部双重微管上。人们对他们定期安排的依据知之甚少。在这项研究中，参与内臂动力蛋白的附件的蛋白质进行了搜索的微管覆盖试验衣原体突变体轴丝。在突变体ida 6和pp中发现一个58 kDa的蛋白质（p58）减少了80%，这两个突变体都缺少7个内臂种类（a-g）中的一个（种类e）。其cDNA的分析表明，p58与tektin同源，tektin是一种最初在海胆中发现的蛋白质，被认为对双微管的纵向周期性至关重要。不像海胆tektin，这是一个组件的原丝丝带，发生后Sarkosyl治疗的轴丝，p58不包含在类似Sarkosyl耐带状衣原体轴丝。免疫荧光显示p58沿着轴丝和基体均匀分布。在ida 6和pp中p58信号减弱。这些结果表明，减少量的p58是足够的生产外双峰，而额外的量，它是参与内臂动力蛋白附着。
In ciliary and flagellar axonemes, various discrete structures such as inner and outer dynein arms are regularly arranged on the outer doublet microtubules. Little is known about the basis for their regular arrangement. In this study, proteins involved in the attachment of inner-arm dyneins were searched by a microtubule overlay assay on Chlamydomonas mutant axonemes. A 58-kDa protein (p58) was found similar to80% diminished in the mutants ida6 and pp, both lacking one (species e) of the seven inner-arm species (a-g). Analysis of its cDNA indicated that p58 is homologous to tektin, a protein that was originally found in sea urchin and thought to be crucial for the longitudinal periodicity of the doublet microtubule. Unlike sea urchin tektin, which is a component of protofilament ribbons that occur after Sarkosyl treatment of axonemes, p58 was not contained in similar Sarkosyl-resistant ribbons from Chlamydomonas axonemes. Immunofluorescence microscopy showed that p58 was localized uniformly along the axoneme and on the basal body. The p58 signal was reduced in ida6 and pp. These results suggest that a reduced amount of p58 is sufficient for the production of outer doublets, whereas an additional amount of it is involved in inner-arm dynein attachment.