Nucleus raphe dorsalis: A morphometric golgi study in rats of three age groups
Nucleus raphe dorsalis: A morphometric golgi study in rats of three age groups
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中缝背核：三个年龄组大鼠的形态测量高尔基体研究
DOI：
10.1016/0006-8993(81)90675-2
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发表时间：
1981
期刊：
影响因子：
2.9
通讯作者：
P. Morgane
中科院分区：
文献类型：
--
作者：
S. Dı́az;L. Cintra;T. Kemper;O. Resnick;P. Morgane
利用快速高尔基-尼氏染色技术，在30、90和220日龄的雄性大鼠中缝背核中鉴定出三种主要的细胞类型：梭形、多极和回避形。我们已经描述了中缝细胞的方向和树突状结构，以及这些细胞与血管和周围结构的关系。对于每种细胞类型，轴突的起源是有特征的。每个年龄组100个神经元在其最大线性范围进行测量，并对体表面上的棘的数量进行计数。对每个年龄组相同数量的一级和二级树突中的树突数量、线性范围、直径和沿树突长度中点附近沿着50 μm段的棘数进行定量。在多极和回避形细胞中，最显著的年龄相关变化是树突数量、直径和棘数量以及体周棘数量。梭形细胞的年龄相关性变化最小。一般来说，中缝背核组织为网状核，神经元具有很少、直且分支不良的树突。
Using Rapid Golgi Nissl techniques, three major cell types: fusiform, multipolar and avoid-shaped were identified in the nucleus raphe dorsalis of male rats at 30, 90, and 220 days of age. We have described the orientation and dendritic architecture of raphe cells as to type and the relationships of these cells to blood vessels and sorrounding structures. For each cell type, the origin of the axon is characteristic. One hundred neurons per age group were measured at their maximal linear extent and the number of spines on the somal surface was counted. Dendritic number, linear extent, diameter and the number of spines along a 50 μm segment near the mid-point of dendritic lenght in an equal number of primary and secondary dendrites were quantified in each age group. The most striking age-related changes in the multipolar and avoid-shaped cells were dendritic number, diameter and spine number as well as the number of perisomatic spines. The fusiform cells showed the least age-related changes. In general, the nucleus raphe dorsalis is organized as a reticular nucleus with neurons having few, straight and poorly ramified dendrites.