A further phylogenetic study of the heterotrophic dinoflagellate genus, Protoperidinium (Dinophyceae) based on small and large subunit ribosomal RNA gene sequences
A further phylogenetic study of the heterotrophic dinoflagellate genus, Protoperidinium (Dinophyceae) based on small and large subunit ribosomal RNA gene sequences
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DOI：
10.1111/j.1440-1835.2006.00438.x
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发表时间：
2006-12
影响因子：
1.5
通讯作者：
Aika Yamaguchi;H. Kawamura;T. Horiguchi
中科院分区：
文献类型：
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作者：
Aika Yamaguchi;H. Kawamura;T. Horiguchi
异养海洋甲藻属原甲藻属是甲藻纲中最大的属。此前，我们基于小亚基 (SSU) rDNA 序列报道了来自四个部分的 10 个 Protoperidinium 物种的属内和属间系统发育关系。本论文报告了来自另外 5 个物种的数据的影响，因此，另外两个部分使用 SSU rDNA 数据，但现在还纳入了来自大亚基 (LSU) rDNA 的序列数据。这些序列，单独或组合，被用来重建该属的进化历史。 LSU rDNA 树支持单系属，但甲藻纲内的系统发育位置仍然不明确。 SSU、LSU 和 SSU + LSU rDNA 系统发育支持 Avellana、Divergentia、Oceanica 和 Protoperidinium 组中的单系，但 Conica 组是并系系。因此，基于 1' 板和 2a 板形状的离散分类部分的概念得到了分子系统发育学的支持。此外，Oceanica 组与该属内的其他类群早有分歧。 Avellana 和 Excentrica 部分以及独立衍生自 Conica 型甲藻的 Divergentia/Protoperidinium 部分的分支。对 LSU rDNA 数据的分析得出了与使用 SSU rDNA 数据获得的系统发育相同的结果，并且随着分类单元采样的增加（包括新部分的成员），获得了对 Protoperidinium 属内形态特征进化的更清晰的认识。基于 SSU rDNA 数据，在 Protoperidinium conicum (Gran) Balech、Protoperidinium excentricum (Paulsen) Balech 和 Protoperidinium pellucidum Bergh 中发现了种内变异，并且在 Protoperidinium claudicans (Paulsen) Balech、P. conicum 和 Protoperidinium denticulatum (Gran et Braarud) Balech 中也发现了基于 LSU rDNA 序列的种内变异。 P. conicum 中常见的碱基对取代表明存在隐秘物种。
The heterotrophic marine dinoflagellate genus Protoperidinium is the largest genus in the Dinophyceae. Previously, we reported on the intrageneric and intergeneric phylogenetic relationships of 10 species of Protoperidinium, from four sections, based on small subunit (SSU) rDNA sequences. The present paper reports on the impact of data from an additional 5 species and, therefore, an additional two sections, using the SSU rDNA data, but now also incorporating sequence data from the large subunit (LSU) rDNA. These sequences, in isolation and in combination, were used to reconstruct the evolutionary history of the genus. The LSU rDNA trees support a monophyletic genus, but the phylogenetic position within the Dinophyceae remains ambiguous. The SSU, LSU and SSU + LSU rDNA phylogenies support monophyly in the sections Avellana, Divergentia, Oceanica and Protoperidinium, but the section Conica is paraphyletic. Therefore, the concept of discrete taxonomic sections based on the shape of 1′ plate and 2a plate is upheld by molecular phylogeny. Furthermore, the section Oceanica is indicated as having an early divergence from other groups within the genus. The sections Avellana and Excentrica and a clade combining the sections Divergentia/Protoperidinium derived from Conica‐type dinoflagellates independently. Analysis of the LSU rDNA data resulted in the same phylogeny as that obtained using SSU rDNA data and, with increased taxon sampling, including members of new sections, a clearer idea of the evolution of morphological features within the genus Protoperidinium was obtained. Intraspecific variation was found in Protoperidinium conicum (Gran) Balech, Protoperidinium excentricum (Paulsen) Balech and Protoperidinium pellucidum Bergh based on SSU rDNA data and also in Protoperidinium claudicans (Paulsen) Balech, P. conicum and Protoperidinium denticulatum (Gran et Braarud) Balech based on LSU rDNA sequences. The common occurrence of base pair substitutions in P. conicum is indicative of the presence of cryptic species.