Therapeutic Effect of Mongolian Medicine RuXian-I on Hyperplasia of Mammary Gland Induced by Estrogen/Progesterone through CRYAB-Promoted Apoptosis
Therapeutic Effect of Mongolian Medicine RuXian-I on Hyperplasia of Mammary Gland Induced by Estrogen/Progesterone through CRYAB-Promoted Apoptosis
复制标题
蒙药乳仙一号通过CRYAB促进细胞凋亡对雌孕激素所致乳腺增生的治疗作用
DOI：
10.1155/2020/5707106
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发表时间：
2020-05-27
影响因子：
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通讯作者：
Wei, Cheng-Xi
中科院分区：
文献类型：
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作者：
Liu, Jia;Zhang, Jun-Fei;Wei, Cheng-Xi
蒙药乳仙I号是根据蒙医的基本原理，在临床上专用于治疗乳腺增生症的经验方，但其作用机制尚不完全清楚。本文从毒性和活性两个方面阐述了乳仙I号治疗雌孕激素所致HMG的作用机制。首先，乳仙1号对HMG大鼠无毒性作用，体重和摄食量无明显变化，心、肝、脾、肺、肾等脏器未见病理改变。其次，乳仙I号能显著降低HMG大鼠乳头高度和直径的增长，显著减轻HMG大鼠的病理改变，并能降低HMG大鼠的血清性激素水平(雌二醇(E-2)、黄体生成素(LH)、孕酮(P)和睾酮(T))。如仙I号通过上调促凋亡蛋白Caspase-3、8、9、Bax和抑癌蛋白P53，降低抗凋亡蛋白Bcl2，释放细胞色素c，明显抑制HMG大鼠乳腺抗凋亡蛋白CryAB的表达，促进HMG大鼠乳腺细胞的凋亡。提示乳仙I号对雌激素和孕激素诱导的HMG大鼠具有保护和治疗作用，其机制可能与促进CryAB调控的细胞凋亡途径有关，是治疗HMG的有效药物。
The traditional Mongolian medicine (TMM) RuXian-I is an empirical formula specifically used for treating the hyperplasia of mammary gland (HMG) in clinic based on the principles of traditional Mongolian medicine, but the treatment mechanism is not completely clear. In this paper, we elaborated the mechanism of RuXian-I in the treatment of HMG induced by estrogen and progestogen from its toxicity and activity. Firstly, RuXian-I exhibited no toxic effect on HMG rats through no changes of body weight and food intake measurement and no pathologic changes of the organs (heart, liver, spleen, lung, and kidney) detected. Secondly, RuXian-I could decrease the increased nipple height and diameter and remarkably relieve the pathologic changes of HMG rats and also alleviate serum sex hormone levels (estradiol (E-2), luteinizing hormone (LH), progesterone (P), and testosterone (T)) of HMG rats. Finally, RuXian-I could obviously inhibit the upregulation level of antiapoptotic protein CRYAB of HMG rats and promote mammary gland cell apoptosis of HMG rats via increases of promoting apoptosis protein caspases-3, 8, and 9 and Bax and tumor suppressor protein p53, decreases of antiapoptosis protein Bcl-2, and release of cytochrome c. These results suggested that RuXian-I has protective and therapeutic effects on HMG rats induced by estrogen and progestogen possibly via promoting apoptotic pathway regulated by CRYAB and is a promising agent for treating HMG.