A gene-stacking approach to overcome the trade-off between drought stress tolerance and growth in Arabidopsis
A gene-stacking approach to overcome the trade-off between drought stress tolerance and growth in Arabidopsis
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DOI：
10.1111/tpj.14110
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发表时间：
2019-01-01
期刊：
影响因子：
7.2
通讯作者：
Yamaguchi-Shinozaki, Kazuko
中科院分区：
文献类型：
--
作者：
Kudo, Madoka;Kidokoro, Satoshi;Yamaguchi-Shinozaki, Kazuko
耐旱作物的分子育种是稳定粮食和生物质生产的关键。然而，在植物生长和耐旱性之间存在着权衡。许多高表达胁迫诱导基因的耐旱植物，如脱水反应元件结合蛋白1A(DREB1A)，表现出严重的生长迟缓。在这里，我们证明了通过共表达在干旱胁迫条件下表达抑制的生长促进基因，可以促进高表达DREB1A的拟南芥植株的生长。我们使用了拟南芥GA Required 5(GA5)和光敏色素相互作用因子4(PIF4)来促进生长，GA5是赤霉素生物合成酶的限速基因，而光敏色素相互作用因子4是编码调节细胞生长对光和温度的反应的转录因子。我们观察到GA5DREB1A和PIF4DREB1A双过表达基因的生物量和成花诱导作用高于DREB1A超表达基因。尽管GA5 DREB1A双过表达基因继续表现出较高的抗旱性，但PIF4 DREB1A双过表达基因由于DREB1A基因表达被抑制而表现出比DREB1A超表达基因更低的胁迫耐性。对GA5和DREB1A双过表达基因的组学分析表明，GA5和DREB1A的共表达对植物的初级代谢、基因表达和植物激素谱产生了额外的影响。这些多方向的分析表明，植物生长和干旱胁迫耐性之间的内在权衡可以通过适当的基因堆积方法来克服。本研究为利用转基因技术改善耐旱植物的生长，稳定粮食和生物量的生产提供了依据。
The molecular breeding of drought stress-tolerant crops is imperative for stable food and biomass production. However, a trade-off exists between plant growth and drought stress tolerance. Many drought stress-tolerant plants overexpressing stress-inducible genes, such as DEHYDRATION-RESPONSIVE ELEMENT-BINDING PROTEIN 1A (DREB1A), show severe growth retardation. Here, we demonstrate that the growth of DREB1A-overexpressing Arabidopsis plants could be improved by co-expressing growth-enhancing genes whose expression is repressed under drought stress conditions. We used Arabidopsis GA REQUIRING 5 (GA5), which encodes a rate-limiting gibberellin biosynthetic enzyme, and PHYTOCHROME-INTERACTING FACTOR 4 (PIF4), which encodes a transcription factor regulating cell growth in response to light and temperature, for growth improvement. We observed an enhanced biomass and floral induction in the GA5 DREB1A and PIF4 DREB1A double overexpressors compared with those in the DREB1A overexpressors. Although the GA5 DREB1A double overexpressors continued to show high levels of drought stress tolerance, the PIF4 DREB1A double overexpressors showed lower levels of stress tolerance than the DREB1A overexpressors due to repressed expression of DREB1A. A multiomics analysis of the GA5 DREB1A double overexpressors showed that the co-expression of GA5 and DREB1A additively affected primary metabolism, gene expression and plant hormone profiles in the plants. These multidirectional analyses indicate that the inherent trade-off between growth and drought stress tolerance in plants can be overcome by appropriate gene-stacking approaches. Our study provides a basis for using genetic modification to improve the growth of drought stress-tolerant plants for the stable production of food and biomass.