An ankyrin-repeat and WRKY-domain-containing immune receptor confers stripe rust resistance in wheat
An ankyrin-repeat and WRKY-domain-containing immune receptor confers stripe rust resistance in wheat
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DOI：
10.1038/s41467-020-15139-6
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发表时间：
2020-03-13
影响因子：
16.6
通讯作者：
Tang, Dingzhong
中科院分区：
文献类型：
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作者：
Wang, Huan;Zou, Shenghao;Tang, Dingzhong
植物中致病效应物的感知通常依赖于核苷酸结合结构域（NBS）和富含亮氨酸重复序列（NLR）蛋白。一些NLR含有额外的结构域，其作为病原体效应物靶标的整合诱饵和免疫信号传导的激活。小麦条锈病是危害农作物最严重的病害之一。在这里，我们报告的YrU 1，一个抗条锈病基因的二倍体小麦乌拉尔图，六倍体小麦的A基因组的祖先的克隆。YrU 1编码一种富含NBS-leucine的卷曲螺旋重复蛋白，具有N端锚定重复和C端WRKY结构域，代表了植物中独特的NLR结构。数据库搜索仅在小麦近缘种中发现了类似的结构。本氏烟草中YrU 1的瞬时表达在没有病原体的情况下不诱导细胞死亡。YrU 1的锚蛋白重复序列和卷曲螺旋结构域自我结合，表明同二聚化对于YrU 1功能至关重要。该抗病基因的鉴定和克隆为进一步研究NLR蛋白的功能奠定了基础，为小麦条锈病的防治提供了重要的育种依据。
Perception of pathogenic effectors in plants often relies on nucleotide-binding domain (NBS) and leucine-rich-repeat-containing (NLR) proteins. Some NLRs contain additional domains that function as integrated decoys for pathogen effector targets and activation of immune signalling. Wheat stripe rust is one of the most devastating diseases of crop plants. Here, we report the cloning of YrU1, a stripe rust resistance gene from the diploid wheat Triticum urartu, the progenitor of the A genome of hexaploid wheat. YrU1 encodes a coiled-coil-NBS-leucine-rich repeat protein with N-terminal ankyrin-repeat and C-terminal WRKY domains, representing a unique NLR structure in plants. Database searches identify similar architecture only in wheat relatives. Transient expression of YrU1 in Nicotiana benthamiana does not induce cell death in the absence of pathogens. The ankyrin-repeat and coiled-coil domains of YrU1 self-associate, suggesting that homodimerisation is critical for YrU1 function. The identification and cloning of this disease resistance gene sheds light on NLR protein function and may facilitate breeding to control the devastating wheat stripe rust disease.