Nasal interleukin 25 as a novel biomarker for patients with chronic rhinosinusitis with nasal polyps and airway hypersensitiveness A pilot study
Nasal interleukin 25 as a novel biomarker for patients with chronic rhinosinusitis with nasal polyps and airway hypersensitiveness A pilot study
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DOI：
10.1016/j.anai.2017.07.012
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发表时间：
2017-10-01
影响因子：
5.9
通讯作者：
Shi, Jianbo
中科院分区：
文献类型：
--
作者：
Chen, Fenghong;Hong, Haiyu;Shi, Jianbo
背景资料：慢性鼻窦炎伴鼻息肉（CRSwNP）是一种上气道慢性炎症性疾病，与气道高反应性（AHR）和哮喘密切相关。目的：探讨CRSwNP患者AHR和哮喘的替代生物标志物（哮喘，n = 17;无症状AHR，n = 11;非AHR，n = 14），11例慢性鼻窦炎无鼻息肉（CRSsNP）患者和13例对照。采用实时定量聚合酶链反应（RT-PCR）和多重免疫分析法分别检测鼻息肉（NP）和对照鼻窦组织中白细胞介素（IL）25和其他细胞因子的蛋白和信使RNA水平。采用多因素Logistic回归和受试者工作特征曲线分析IL-25的临床相关性。结果：与对照组、CRSsNP组和正常对照组相比，CRSsNP组鼻黏膜组织中IL-25的蛋白和mRNA水平均显著升高。多因素Logistic回归分析显示鼻IL-25蛋白水平、鼻及血嗜酸性粒细胞计数是CRSwNP患者AHR的独立危险因素。根据受试者工作特征曲线分析，鼻组织IL-25检测的敏感性为91.4%，特异性为62.8%（曲线下面积，0.845），用于指示该CRSwNP组群中的AHR。我们的研究结果表明，IL-25在NP组织中显著升高，可以被认为是CRSwNP患者AHR的分子指标。(C)2017年美国过敏，哮喘和免疫学学院。爱思唯尔公司出版All rights reserved.
Background: Chronic rhinosinusitis with nasal polyps (CRSwNP) is a chronic inflammatory disease of the upper airway and is tightly linked with airway hyperresponsiveness (AHR) and asthma. However, the surrogate biomarkers for indicating AHR and asthma in patients with CRSwNP remain elusive.Objective: To investigate the surrogate biomarkers for indicating AHR and asthma in patients with CRSwNP.Methods: In this study, sinonasal tissues were collected from 42 patients with CRSwNP (asthma, n = 17; asymptomatic AHR, n = 11; non-AHR, n = 14), 11 patients with chronic rhinosinusitis without nasal polyps (CRSsNP), and 13 controls. The protein and messenger RNA levels of interleukin (IL) 25 and other cytokines in nasal polyp (NP) and control sinonasal tissues were determined by quantitative real-time polymerase chain reaction and multiplex immunoassay, respectively. Multivariate logistic regression and receiver operating characteristic curve analysis were performed to assess the clinical relevance of IL-25.Results: We found that the protein and messenger RNA levels of IL-25 were significantly increased in NP tissues compared with the control sinonasal tissues from patients with CRSwNP, patients with CRSsNP, and controls. Multivariate logistic regression revealed that the nasal IL-25 protein level and nasal and blood eosinophil counts were independent risk factors for AHR in patients with CRSwNP. According to receiver operating characteristic curve analysis, nasal tissue IL-25 had a sensitivity of 91.4% and a specificity of 62.8% (area under the curve, 0.845) at the cutoff level of 5 pg/mu L for indicating AHR in this CRSwNP cohort.Conclusion: Our findings indicated that IL-25 was significantly increased in NP tissues and may be considered as the molecular indicator for AHR in patients with CRSwNP. (C) 2017 American College of Allergy, Asthma & Immunology. Published by Elsevier Inc. All rights reserved.