Plasma appearance and disappearance of an oral dose of 25-hydroxyvitamin D2 in healthy adults.
Plasma appearance and disappearance of an oral dose of 25-hydroxyvitamin D2 in healthy adults.
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DOI：
10.1017/s0007114511004132
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发表时间：
2012-04
影响因子：
3.6
通讯作者：
Prentice, Ann
中科院分区：
文献类型：
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作者：
Jones, Kerry S.;Schoenmakers, Inez;Bluck, Les J. C.;Ding, Shujing;Prentice, Ann
25-羟基维生素 D (25(OH)D) 半衰期是研究维生素 D 代谢和需求的潜在生物标志物。我们进行了一项初步研究，以评估口服剂量后测量 25(OH)D 半衰期的方法和实际可行性。总共 12 名 18-23 岁的健康冈比亚男性被分为两组，以研究口服 80 nmol 25(OH)D2 剂量后 (1) 吸收和 (2) 血浆消失的速率和时间。在基线时收集空腹血样，第一组在给药后 12 小时、24 小时、48 小时和第 15 天每 2 小时收集一次空腹血样。在第二组中，在第 3、4、5、6、9、12、15、18 和 21 天收集空腹血样。在基线和第 15 天第一次早晨排尿后收集尿液 2 小时。通过超高效液相色谱-串联 MS/MS 测量 25(OH)D2 血浆浓度并校正基线。在基线和第 15 天测量维生素 D、Ca 和 P 代谢的生物标志物。4·4 (sd 1·8) h 时 25(OH)D2 的血浆峰值浓度为 9·6 (sd 0·9) nmol/l。确定 25(OH)D2 消失的终末斜率从第 6 天开始。血浆 25(OH)D2 的终末半衰期为 13·4 (sd 2·7) d。基线和第 15 天之间以及两组之间的血浆 25(OH)D3、总 1,25(OH)2D、甲状旁腺激素、P、Ca 和离子化 Ca 以及尿 Ca 和 P 没有显着差异。本研究提供了口服 25(OH)D2 的血浆反应数据，这将支持并有助于进一步开展 25(OH)D 半衰期研究。
25-Hydroxyvitamin D (25(OH)D) half-life is a potential biomarker for investigating vitamin D metabolism and requirements. We performed a pilot study to assess the approach and practical feasibility of measuring 25(OH)D half-life after an oral dose. A total of twelve healthy Gambian men aged 18–23 years were divided into two groups to investigate the rate and timing of (1) absorption and (2) plasma disappearance after an 80 nmol oral dose of 25(OH)D2. Fasting blood samples were collected at baseline and, in the first group, every 2 h post-dose for 12 h, at 24 h, 48 h and on day 15. In the second group, fasting blood samples were collected on days 3, 4, 5, 6, 9, 12, 15, 18 and 21. Urine was collected for 2 h after the first morning void at baseline and on day 15. 25(OH)D2 plasma concentration was measured by ultra-performance liquid chromatography-tandem MS/MS and corrected for baseline. Biomarkers of vitamin D, Ca and P metabolism were measured at baseline and on day 15. The peak plasma concentration of 25(OH)D2 was 9·6 (sd 0·9) nmol/l at 4·4 (sd 1·8) h. The terminal slope of 25(OH)D2 disappearance was identified to commence from day 6. The terminal half-life of plasma 25(OH)D2 was 13·4 (sd 2·7) d. There were no significant differences in plasma 25(OH)D3, total 1,25(OH)2D, parathyroid hormone, P, Ca and ionised Ca and urinary Ca and P between baseline and day 15 and between the two groups. The present study provides data on the plasma response to oral 25(OH)D2 that will underpin and contribute to the further development of studies to investigate 25(OH)D half-life.