Maternal diesel exhaust exposure damages newborn murine brains
Maternal diesel exhaust exposure damages newborn murine brains
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DOI：
10.1248/jhs.52.82
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发表时间：
2006-02-01
影响因子：
--
通讯作者：
Takeda, K
中科院分区：
文献类型：
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作者：
Sugamata, M;Ihara, T;Takeda, K
为了从病理学上研究柴油机尾气（DE）暴露对胎鼠神经系统发育的影响，收集了妊娠期暴露于DE的母鼠的新生小鼠脑组织（大脑皮质和海马）。DE暴露后，这些脑组织显示出大量caspase 3阳性细胞（细胞凋亡的常见酶生物标志物）的证据。一些细胞被发现含有新月形的空间，这是凋亡过程的暗示。颗粒周皮（GP）细胞，血脑屏障脑血管周围的清道夫细胞，显示出凋亡的迹象;此外，GP细胞质颗粒已经退化，并显示出什么似乎是超细，DE颗粒的证据。此外，毛细血管周围的星形胶质细胞足端肿胀显示出类似于髓鞘的退行性变化。此外，还观察到内皮细胞凋亡和部分毛细血管狭窄。这些发现的严重程度各不相同，取决于DE浓度，在对照组中未观察到。这些观察结果表明，妊娠小鼠暴露于DE可能具有细胞萎缩的风险，并可能影响胎儿大脑发育。我们的研究结果还表明，吸入DE可能对胎儿的一般健康有害。
To examine pathologically the influence of diesel exhaust (DE) exposure on fetal nervous system development, brain tissue (cerebral cortex and hippocampus) was collected from newborn mice whose mothers were exposed to DE during pregnancy. After DE exposure, these brain tissues showed evidence of numerous caspase 3-positive cells (a common enzymatic biomarker of apoptosis). Some cells were found to contain crescent-shaped spaces, which are suggestive of apoptotic processes. Granular perithelial (GP) cells, scavenger cells surrounding cerebral vessels of the blood-brain barrier, showed signs of apoptosis; furthermore, the GP cytoplasmic granules had degenerated and showed evidence of what appeared to be ultrafine, DE particles. Additionally, the swelling of astrocyte endfoot that surround capillaries showed degenerative changes similar to myelin figures. Furthermore, the apoptosis of endothelial cells and stenosis of some capillaries were observed. These findings varied in severity, depended on DE concentration, and were not observed in the control group. These observations suggest that exposure of pregnant mice to DE might carry a risk of cellular atrophy and might affect fetal brain development. Our findings also reveal that inhalation of DE might be hazardous to the general health of fetuses.