Cooperation of endoderm-derived BMP2 and extraembryonic ectoderm-derived BMP4 in primordial germ cell generation in the mouse.
Cooperation of endoderm-derived BMP2 and extraembryonic ectoderm-derived BMP4 in primordial germ cell generation in the mouse.
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DOI：
10.1006/dbio.2001.0173
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发表时间：
2001-04
影响因子：
2.7
通讯作者：
Ying Ying-Ying;G. Zhao
中科院分区：
文献类型：
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作者：
Ying Ying-Ying;G. Zhao
小鼠的原始生殖细胞（PGCs）来源于原肠胚形成过程中与胚胎外胚层相邻的近端外胚层细胞。先前的研究表明，胚胎外外胚层来源的BMP4和BMP8B都是PGC生成所必需的。我们发现Bmp超家族Dpp类的成员Bmp2也在PGC的产生中起作用。在C57BL/6背景下，N2代Bmp2杂合子和纯合子胚的PGC数量显著减少。Bmp2纯合子胚胎的尿囊也很短，约50%的胚胎不能进行正常的绒毛膜-尿囊融合。通过原位杂交，我们发现Bmp2主要在小鼠前胚和原胚的内胚层中表达。利用遗传方法，我们进一步表明Bmp2和Bmp4，而不是Bmp2和Bmp8b，对PGC的产生有加性作用。这些结果表明，小鼠胚胎中PGC的产生不仅受胚胎外外胚层来源的BMP4和BMP8B的调控，还受内胚层来源的BMP2的调控。
The primordial germ cells (PGCs) of the mouse are derived from proximal epiblast cells that are adjacent to the extraembryonic ectoderm during gastrulation. Previous studies have demonstrated that extraembryonic ectoderm-derived BMP4 and BMP8B are both required for PGC generation. Here we show that Bmp2, a member of the Dpp class of the Bmp superfamily, also plays a role in PGC generation. PGC number is significantly reduced in Bmp2 heterozygous and homozygous embryos at the N2 generation onto C57BL/6 background. Bmp2 homozygous embryos also have a short allantois and about 50% of them do not undergo normal chorioallantoic fusion. Using whole-mount in situ hybridization, we show that Bmp2 is primarily expressed in the endoderm of mouse pregastrula and gastrula embryos. Using a genetic approach, we further show that Bmp2 and Bmp4, but not Bmp2 and Bmp8b, have an additive effect on PGC generation. These results suggest that PGC generation in the mouse embryo is regulated not only by extraembryonic ectoderm-derived BMP4 and BMP8B, but also by endoderm-derived BMP2.