Cadaver validation of skeletal muscle measurement by magnetic resonance imaging and computerized tomography
Cadaver validation of skeletal muscle measurement by magnetic resonance imaging and computerized tomography
复制标题
DOI：
10.1152/jappl.1998.85.1.115
复制
发表时间：
1998-07-01
影响因子：
3.3
通讯作者：
Ross, R
中科院分区：
文献类型：
--
作者：
Mitsiopoulos, N;Baumgartner, RN;Ross, R
磁共振成像（MRI）和计算机断层扫描（CT）是量化全身和局部骨骼肌质量的有前途的参考方法。早期的MRI和CT验证研究使用的数据采集技术和数据分析程序现在已经过时，评估解剖而不是无脂肪组织骨骼肌（ATFSM），只研究相对较大的大腿，或发现过大的估计误差。本研究的目的是通过使用标准MRI和CT采集和图像分析方法与相应的尸体估计值比较手臂和腿部ATFSM横截面积估计值（cm（2））。第二个目的是验证嵌入肌肉（间质脂肪组织）和周围肌肉（皮下脂肪组织）的脂肪组织的MRI和CT测量。通过MRI测量的ATFSM面积（n = 119）[38.9 +/- 22.3（SD）cm（2）]，CT（39.7 +/- 22.8 cm（2））和尸体（39.5 +/- 23.0 cm（2））无差异（P > 0.001），并且ATFSM的MRI和CT估计值与相应的尸体值高度相关[MRI：r = 0.99，估计SE（SEE）3.9 cm（2），P < 0.001; CT：r = 0.99，SEE = 3.8 cm（2），P < 0.001]。在MRI和CT测量的间质和皮下脂肪组织与尸体测量的间质和皮下脂肪组织之间观察到类似的良好结果。对于MRI-ATFSM，体内重复测量的观察者内相关性为0.99 [SEE = 8.7 cm（2）（2.9%），P < 0.001]。这些发现强烈支持使用MRI和CT作为体内阑尾骨骼肌、间质和皮下脂肪组织测量的参考方法。
Magnetic resonance imaging (MRI) and computerized tomography (CT) are promising reference methods for quantifying whole body and regional skeletal muscle mass. Earlier MRI and CT validation studies used data-acquisition techniques and data-analysis procedures now outdated, evaluated anatomic rather than adipose tissue-free skeletal muscle (ATFSM), studied only the relatively large thigh, or found unduly large estimation errors. The aim of the present study was to compare arm and leg ATFSM cross-sectional area estimates (cm(2)) by using standard MRI and CT acquisition and image-analysis methods with corresponding cadaver estimates. A second objective was to validate MRI and CT measurements of adipose tissue embedded within muscle (interstitial adipose tissue) and surrounding muscle (subcutaneous adipose tissue). ATFSM area (n = 119) by MRI [38.9 +/- 22.3 (SD) cm(2)], CT (39.7 +/- 22.8 cm(2)), and cadaver (39.5 +/- 23.0 cm(2)) were not different (P > 0.001), and both MRI and CT estimates of ATFSM were highly correlated with corresponding cadaver values [MRI: r = 0.99, SE of estimate (SEE) 3.9 cm(2), P < 0.001; and CT: r = 0.99, SEE = 3.8 cm2, P < 0.001]. Similarly good results were observed between MRI- and CT-measured vs. cadaver-measured interstitial and subcutaneous adipose tissue. For MRI-ATFSM the intraobserver correlation for duplicate measurements in vivo was 0.99 [SEE = 8.7 cm(2) (2.9%), P < 0.001]. These findings strongly support the use of MRI and CT as reference methods for appendicular skeletal muscle, interstitial and subcutaneous adipose tissue measurement in vivo.