The correlation between microRNA490-3p and TGF alpha in endometrial carcinoma tumorigenesis and progression
The correlation between microRNA490-3p and TGF alpha in endometrial carcinoma tumorigenesis and progression
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DOI：
10.18632/oncotarget.7061
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发表时间：
2016
期刊：
影响因子：
--
通讯作者：
Zhao Yang
中科院分区：
文献类型：
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作者：
Sun Kai-Xuan;Chen Ying;Chen Shuo;Liu Bo-Liang;Feng Miao-Xiao;Zong Zhi-Hong;Zhao Yang
MicroRNAs (miRNAs) are small non-coding RNAs that negatively regulate the translation of messenger RNAs by binding their 3′-untranslated region (3′ UTR). MiR-490-3p has been reported to be a suppressor in various human cancers; however, little is known about the biological functions of miR-490-3p in endometrial cancer (EC). In our study, we found that MiR-490-3p mRNA expression was significantly lower in ECs than in normal endometrial tissues. MiR-490-3p mRNA expression was also negatively associated with depth of invasion (mucosa vs. muscular and serosa) and lymph node metastasis (negative vs. positive) in EC. MiR-490-3p overexpression reduced proliferation; promoted G1 arrest and apoptosis; suppressed migration and invasion; and reduced TGFα, NF-kB, cyclin D1, survivin, matrix metalloproteinase 2 (MMP2) mRNA and protein expression, and improved Bax mRNA and protein expression. The dual-luciferase reporter assay indicated that miR-490-3p directly targeted TGFα by binding its 3′ untranslated region. MiR-490-3P transfection also suppressed tumor development and TGFα expression (as determined by immunohistochemistry and western blotting) in vivo in the xenograft mouse model. This is the first demonstration that miR-490-3P might act as a suppressor in EC tumorigenesis and progression by targeting TGFα. Our results provide a theoretical basis for the further study on the molecular target for endometrial cancer.
