Seminal vesicle production and secretion of growth hormone into seminal fluid
Seminal vesicle production and secretion of growth hormone into seminal fluid
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DOI：
10.1038/15067
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发表时间：
1999-11-01
影响因子：
46.9
通讯作者：
Pothier, F
中科院分区：
文献类型：
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作者：
Dyck, MK;Gagné, D;Pothier, F
在转基因动物的组织中生产外源蛋白代表了生产治疗性和药物性蛋白质的有效且经济的方法。在这项研究中，我们证明，小鼠P12基因启动子的男性附性腺特异性，可用于产生转基因小鼠，表达人类生长激素（hGH）在他们的精囊上皮。hGH分泌到射精精液中，精囊腔内容物的浓度高达0.5 mg/ml。由于精液是一种体液，可以很容易地连续收集，生产转基因动物表达药物蛋白到他们的精液可以证明是一个可行的替代使用乳腺作为生物反应器。
Production of foreign proteins in the tissues of transgenic animals represents an efficient and economical method of producing therapeutic and pharmaceutical proteins. In this study, we demonstrate that the mouse P12 gene promoter specific to the male accessory sex gland can be used to generate transgenic mice that express human growth hormone (hGH) in their seminal vesicle epithelium. The hGH is secreted into the ejaculated seminal fluids with the seminal vesicle lumen contents containing concentrations of up to 0.5 mg/ml. As semen is a body fluid that can be collected easily on a continuous basis, the production of transgenic animals expressing pharmaceutical proteins into their seminal fluid could prove to be a viable alternative to use of the mammary gland as a bioreactor.