Control of size dispersity of chitosan biopolymer microparticles and nanoparticles to influence vaccine trafficking and cell uptake
Control of size dispersity of chitosan biopolymer microparticles and nanoparticles to influence vaccine trafficking and cell uptake
复制标题
DOI：
10.1002/jbm.a.34153
复制
发表时间：
2012-07-01
影响因子：
4.9
通讯作者：
Mainwaring, David E.
中科院分区：
文献类型：
--
作者：
Al Kobiasi, Mohammad;Chua, Brendon Y.;Mainwaring, David E.
利用结构相关的表面活性剂分子来生成壳聚糖乳液，以提供系统的胶束曲率半径和大小变化。这些组合物提供了对壳聚糖颗粒分散性的精确控制，即根据三个定量分布参数的尺寸分布以及形状分布。这导致了一套从71 nm到3.7微米的颗粒尺寸分布和非常高的颗粒球形度，使得颗粒尺寸的影响可以在两项体内研究中分离出来，这些研究将生物聚合物颗粒尺寸与细胞摄取和向淋巴结的运输联系起来。流式细胞仪和荧光显微镜显示，在24小时内，这三个细胞系优先内化的壳聚糖微颗粒的程度高于纳米颗粒。在活体小鼠模型中，纳米颗粒最初快速输送到淋巴结，排出接种部位，随后进一步缓慢摄取。微粒子以相似的模式流向淋巴结，只是初始放电相似，比纳米粒子少50倍，这表明两种粒子的生理传输特性有很大差异。(C)2012年Wiley期刊，Inc.《生物材料与材料》A部分，2012年。
Structurally related surfactant molecules were exploited to generate chitosan emulsions to provide systematic variation in micelle radii of curvature and size. These compositions provide precise control of chitosan particle dispersity, that is, size distribution according to three quantitative distribution parameters as well as shape distribution. This resulted in a suite of particle size distributions spanning 71 nm to 3.7 mu m and a very high degree of particle sphericity, allowing the influence of particle size to be isolated in two in vivo studies relating biopolymer particle size to cellular uptake and trafficking to lymph nodes. Flow cytometry and fluorescence microscopy indicated that the three cell lines examined preferentially internalized chitosan microparticles to a greater extent than nanoparticles over a 24 h period. In an in vivo mouse model, nanoparticles initially trafficked rapidly to lymph nodes draining the site of inoculation followed by further slower uptake. Microparticles trafficked to the lymph nodes with a similar pattern except that the initial discharge was similar to 50-fold less than that observed with nanoparticles indicating a profound difference in the physiological transport properties of the two particle types. (C) 2012 Wiley Periodicals, Inc. J Biomed Mater Res Part A, 2012.