Expression of growth-factor receptors in normal and regenerating human periodontal cells
Expression of growth-factor receptors in normal and regenerating human periodontal cells
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DOI：
10.1016/s0003-9969(00)00099-6
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发表时间：
2001-03-01
影响因子：
3
通讯作者：
Olsen, I
中科院分区：
文献类型：
--
作者：
Parkar, MH;Kuru, L;Olsen, I
生长因子是一种生物活性的介体，它与靶细胞上的特定受体结合，调节与细胞生长、伤口愈合和再生有关的基因。因此，这些受体的表达对于细胞对这些因子的反应至关重要。本研究的目的是用免疫组织化学和流式细胞术检测生长因子受体在正常牙龈、牙周膜和这些组织来源的细胞中的表达，以及引导组织再生(GTR)后再生组织中的表达。免疫细胞化学检测未见血小板衍生生长因子受体-α(PDGF-Rα)在任何组织中表达，而PDGF-Rβ和转化生长因子-β受体I、II型(转化生长因子-βRI，RII)在再生组织中的表达明显高于牙周组织和牙周韧带组织。表皮生长因子受体(EGF-R)在再生组织中也明显升高，在牙龈上皮细胞中表达强烈，而在牙周膜中不表达。成纤维细胞生长因子受体-I(FGF-RI)和胰岛素样生长因子受体(IGF-R)在牙周膜和牙龈中均未检测到，但在再生组织中有时染色较弱。流式细胞仪(FCM)检测显示，正常牙周组织和牙周膜来源的细胞均表达PDGF-Rβ，而转化生长因子-βRI和RII、成纤维细胞生长因子-RI和胰岛素样生长因子-R仅在部分细胞中表达。相反，没有细胞表达PDGF-Rα或EGF-R。这些观察结果表明，生长因子受体在牙周组织和细胞中的表达存在差异，提示相应的因子可能也不同地参与了牙周创伤的修复和再生。(C)2001爱思唯尔科学有限公司。保留所有权利。
Growth factors are biologically active mediators that bind to specific receptors on target cells and regulate genes involved in cell growth, wound healing and regeneration. The expression of these receptors is thus of fundamental importance for the response of the cells to the factors. The aim here was to examine, using immunohistochemistry and flow cytometry, the expression of growth factor receptors in normal gingiva, periodontal ligament and in cells derived from these tissues, and also in regenerated tissues following guided tissue regeneration (GTR). By immunocytochemistry platelet-derived growth factor receptor-alpha (PDGF-R alpha) was not detected in any of the tissues, whereas the PDGF-R beta and transforming growth factor-beta receptor types I and II (TGF-beta RI, RII) appeared to be upregulated in regenerated tissues compared with gingival and periodontal ligament tissues. Epidermal growth factor receptor (EGF-R) was also notably elevated in the regenerated tissue and was strongly expressed in the gingival epithelium but not in the periodontal ligament. Neither were fibroblast growth factor receptor-I (FGF-RI) or insulin-like growth factor receptor (IGF-R) detected in the periodontal ligament, nor in the gingiva, but they sometimes stained weakly in the regenerated tissues. Flow cytometry (FCM) showed that all the cells derived from the normal gingiva and the periodontal ligament expressed the PDGF-R beta, whereas the TGF-beta RI and RII, FGF-RI and IGF-R were detected in only a proportion of the total cells. In contrast, none of the cells expressed the PDGF-R alpha or the EGF-R. These observations show that the growth factor receptors are differentially expressed by the periodontal tissues and cells and suggest that the corresponding factors may also be differentially involved in periodontal wound healing and regeneration. (C) 2001 Elsevier Science Ltd. All rights reserved.