Non-viral Induction of Transgene-free iPSCs from Somatic Fibroblasts of Multiple Mammalian Species.
Non-viral Induction of Transgene-free iPSCs from Somatic Fibroblasts of Multiple Mammalian Species.
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DOI：
10.1016/j.stemcr.2021.03.002
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发表时间：
2021-04-13
影响因子：
5.9
通讯作者：
Okano H
中科院分区：
文献类型：
--
作者：
Yoshimatsu S;Nakajima M;Iguchi A;Sanosaka T;Sato T;Nakamura M;Nakajima R;Arai E;Ishikawa M;Imaizumi K;Watanabe H;Okahara J;Noce T;Takeda Y;Sasaki E;Behr R;Edamura K;Shiozawa S;Okano H
诱导多能干细胞（iPSC）能够提供来自所有三个胚层和生殖细胞的无限细胞来源。从各种动物模型中衍生和使用iPSC可以促进基于干细胞的治疗，基因修饰的动物生产和评估物种间差异的进化研究。然而，缺乏用于衍生iPSC的物种范围的方法，特别是通过非病毒和非转基因整合（NTI）方法。在这里，我们证明了iPSC衍生自体细胞成纤维细胞的多个哺乳动物物种从三个不同的分类目的，包括常见的绒猴（Callithrix jacchus）在灵长类动物，狗（犬狼疮familiaris）在食肉动物，和猪（猪）在Cetartiodactyla，通过组合使用的化学化合物和NTI附加型载体。有趣的是，成纤维细胞在重编程过程中暂时获得了神经干细胞样状态。总的来说，我们的方法，强大的适用于各种物种，具有很大的潜力，促进干细胞为基础的研究，使用各种动物在哺乳动物。已经建立了一种用于衍生无转基因iPSC的物种范围的方法。原代集落形成细胞显示出神经干细胞样特征，称为iNSLCs。进行mRNA-seq以剖析iNSLCs和iPSC之间的差异。进行单细胞分析以评估iNSLCs的异质性。在这篇文章中，Okano和同事建立了一种用于衍生无转基因iPSC的物种范围的方法，并发现原代集落形成细胞表现出神经干细胞样特征，命名为诱导神经干细胞样细胞（iNSLCs）。
Induced pluripotent stem cells (iPSCs) are capable of providing an unlimited source of cells from all three germ layers and germ cells. The derivation and usage of iPSCs from various animal models may facilitate stem cell-based therapy, gene-modified animal production, and evolutionary studies assessing interspecies differences. However, there is a lack of species-wide methods for deriving iPSCs, in particular by means of non-viral and non-transgene-integrating (NTI) approaches. Here, we demonstrate the iPSC derivation from somatic fibroblasts of multiple mammalian species from three different taxonomic orders, including the common marmoset (Callithrix jacchus) in Primates, the dog (Canis lupus familiaris) in Carnivora, and the pig (Sus scrofa) in Cetartiodactyla, by combinatorial usage of chemical compounds and NTI episomal vectors. Interestingly, the fibroblasts temporarily acquired a neural stem cell-like state during the reprogramming. Collectively, our method, robustly applicable to various species, holds a great potential for facilitating stem cell-based research using various animals in Mammalia. A species-wide method for deriving transgene-free iPSCs has been established Primary colony-forming cells showed a neural stem cell-like profile, named iNSLCs mRNA-seq was performed for dissecting the difference between iNSLCs and iPSCs Single-cell analysis was performed for assessing the heterogeneity of iNSLCs In this article, Okano and colleagues established a species-wide method for deriving transgene-free iPSCs, and discovered primary colony-forming cells showed a neural stem cell-like profile, named induced neural stem cell-like cells (iNSLCs).
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影响因子：
13.6
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Honda A;Choijookhuu N;Izu H;Kawano Y;Inokuchi M;Honsho K;Lee AR;Nabekura H;Ohta H;Tsukiyama T;Ohinata Y;Kuroiwa A;Hishikawa Y;Saitou M;Jogahara T;Koshimoto C
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Koshimoto C
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Hayashi T;Ozaki H;Sasagawa Y;Umeda M;Danno H;Nikaido I
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作者：
Marchetto, Maria C. N.;Narvaiza, Inigo;Denli, Ahmet M.;Benner, Christopher;Lazzarini, Thomas A.;Nathanson, Jason L.;Paquola, Apua C. M.;Desai, Keval N.;Herai, Roberto H.;Weitzman, Matthew D.;Yeo, Gene W.;Muotri, Alysson R.;Gage, Fred H.
通讯作者：
Gage, Fred H.
影响因子：
64.8
作者：
Kobayashi T;Zhang H;Tang WWC;Irie N;Withey S;Klisch D;Sybirna A;Dietmann S;Contreras DA;Webb R;Allegrucci C;Alberio R;Surani MA
通讯作者：
Surani MA
影响因子：
23.9
作者：
Nichols, Jennifer;Smith, Austin
通讯作者：
Smith, Austin