FUT-175 inhibits the production of IL-6 and IL-8 in human monocytes.
FUT-175 inhibits the production of IL-6 and IL-8 in human monocytes.
复制标题
FUT-175 抑制人单核细胞中 IL-6 和 IL-8 的产生。
DOI：
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发表时间：
1999
期刊：
影响因子：
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通讯作者：
M. Ogawa
中科院分区：
文献类型：
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作者：
H. Sugita;T. Ishiko;S. Ikei;M. Hirota;M. Ogawa
我们观察了丝氨酸蛋白酶抑制剂FUT-175对内毒素刺激单核细胞产生促炎细胞因子IL-6和IL-8的影响。分离的健康志愿者单核细胞与内毒素和不同浓度的FUT-175孵育12h。用酶联免疫吸附试验检测，FUT在1~100微g/ml浓度范围内对IL-6和IL-8的产生均有明显的抑制作用，且呈剂量依赖性。此外，FUT-175还能抑制IL-6和IL-8mRNAs的表达。这些数据表明，FUT-175可以减轻与包括IL-6和IL-8在内的促炎细胞因子的增加相关的病理炎症条件。
We investigated the effect of FUT-175, a serine protease inhibitor, on the production of pro-inflammatory cytokines, interleukin-6 (IL-6) and interleukin-8 (IL-8), by monocytes stimulated with lipopolysaccharide (LPS). Monocytes isolated from healthy volunteers were incubated with LPS and various concentrations of FUT-175 for 12 hours. The productions of both IL-6 and IL-8, measured by enzyme-linked immunosorbent assay, were significantly inhibited by FUT at the concentration of 1 to 100 microg/ml in a dose-dependent manner. Furthermore, the expressions of IL-6 and IL-8 mRNAs were also inhibited by FUT-175. These data suggest that FUT-175 may reduce the pathological inflammatory conditions associated with enhanced production of proinflammatory cytokines including IL-6 and IL-8.