Synaptic organization in the adult honey bee brain is influenced by brood-temperature control during pupal development
Synaptic organization in the adult honey bee brain is influenced by brood-temperature control during pupal development
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DOI：
10.1073/pnas.0400773101
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发表时间：
2004-03-23
影响因子：
11.1
通讯作者：
Rössler, W
中科院分区：
文献类型：
--
作者：
Groh, C;Tautz, J;Rössler, W
最近的研究表明，成年蜜蜂的行为表现受到蛹发育过程中所经历的温度的影响。在这里，我们探讨是否有温度介导的影响，对大脑。我们在29到37摄氏度之间的不同恒温下饲养蛹，并对成年人的大脑进行神经解剖学分析。分析集中在蘑菇体的感觉输入区域，与学习和记忆等高阶处理相关的大脑区域。通过使用荧光团缀合的鬼笔环肽和突触蛋白的抗体，蘑菇体肾盏内的独特的突触复合物[微球（MG）]被可视化。在不同温度下饲养的蜜蜂中，IVIG的数量是不同的，这些差异在成年后的第一周仍然存在。在嗅觉输入区（唇），IVIG的数量是最高的蜜蜂饲养在温度通常保持在育雏细胞（34.5 degreesC）和显着下降，在蜜蜂饲养在VC低于和高于这一标准。有趣的是，在邻近的视觉输入区域（衣领），MG数字受温度的影响较小。我们的结论是，温度调节控制育雏饲养可以产生区域和模态特定的影响，在成年大脑中的突触神经末梢。我们建议，在突触电路产生的差异可能会影响神经元的可塑性，并可能根据温度介导的多模态通信和学习的影响。
Recent studies have shown that the behavioral performance of adult honey bees is influenced by the temperature experienced during pupal development. Here we explore whether there are temperature-mediated effects on the brain. We raised pupae at different constant temperatures between 29 and 37degreesC and performed neuroanatomical analyses of the adult brains. Analyses focused on sensory-input regions in the mushroom bodies, brain areas associated with higher-order processing such as learning and memory. Distinct synaptic complexes [microglomeruli (MG)] within the mushroom body calyces were visualized by using fluorophore-conjugated phalloidin and an antibody to synapsin. The numbers of IVIG were different in bees that had been raised at different temperatures, and these differences persisted after the first week of adult life. In the olfactory-input region (lip), IVIG numbers were highest in bees raised at the temperature normally maintained in brood cells (34.5degreesC) and significantly decreased in bees raised at VC below and above this norm. Interestingly, in the neighboring visual-input region (collar), MG numbers were less affected by temperature. We conclude that thermoregulatory control of brood rearing can generate area- and modality-specific effects on synaptic neuropils in the adult brain. We propose that resulting differences in the synaptic circuitry may affect neuronal plasticity and may underlie temperature-mediated effects on multimodal communication and learning.