Isolation and characterization of human apolipoprotein M-containing lipoproteins
Isolation and characterization of human apolipoprotein M-containing lipoproteins
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DOI：
10.1194/jlr.m600055-jlr200
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发表时间：
2006-08-01
影响因子：
6.5
通讯作者：
Dahlback, Bjorn
中科院分区：
文献类型：
--
作者：
Christoffersen, Christina;Nielsen, Lars Bo;Dahlback, Bjorn
载脂蛋白M（apoM）是一种新的载脂蛋白，其功能尚不清楚。本研究建立了一种分离含apoM的脂蛋白的方法，并研究了其组成及apoM对HDL功能的影响。用免疫亲和层析法从人血浆中分离出含载脂蛋白的脂蛋白，并与缺乏载脂蛋白的脂蛋白进行比较。含apoM的脂蛋白主要是HDL大小的;类似于总HDL人群的5%含有apoM。质谱分析显示，含apoM的脂蛋白还含有apoJ、apoA-I、apoA II、apoC-I、apoC-II、apoC-III、对氧磷酶1和apoB。含有apoM的HDL（HDLapoM+）比缺乏apoM的HDL（HDLapoM-）含有显著更多的游离胆固醇（5.9 +/- 0.7%对3.2 +/- 0.5%; P < 0.005），并且大小不均匀，有小颗粒和大颗粒。HDLapoM+比HDLapoM-更有效地抑制Cu 2+诱导的LDL氧化和刺激THP-1泡沫细胞的胆固醇流出。总之，我们的研究结果表明，载脂蛋白M与一个小的异质亚群的HDL颗粒。然而，apoM指定HDL的亚群，其保护LDL免受氧化，并且比缺乏apoM的HDL更有效地刺激胆固醇流出。
Apolipoprotein M (apoM) is a novel apolipoprotein with unknown function. In this study, we established a method for isolating apoM-containing lipoproteins and studied their composition and the effect of apoM on HDL function. ApoM-containing lipoproteins were isolated from human plasma with immunoaffinity chromatography and compared with lipoproteins lacking apoM. The apoM-containing lipoproteins were predominantly of HDL size; similar to 5% of the total HDL population contained apoM. Mass spectrometry showed that the apoM-containing lipoproteins also contained apoJ, apoA-I, apoA II, apoC-I, apoC-II, apoC-III, paraoxonase 1, and apoB. ApoM-containing HDL (HDLapoM+) contained significantly more free cholesterol than HDL lacking apoM (HDLapoM-) (5.9 +/- 0.7% vs. 3.2 +/- 0.5%; P < 0.005) and was heterogeneous in size with both small and large particles. HDLapoM+ inhibited Cu2+-induced oxidation of LDL and stimulated cholesterol efflux from THP-1 foam cells more efficiently than HDLapoM-. In conclusion, our results suggest that apoM is associated with a small heterogeneous subpopulation of HDL particles. Nevertheless, apoM designates a subpopulation of HDL that protects LDL against oxidation and stimulates cholesterol efflux more efficiently than HDL lacking apoM.