Genome-wide identification and evolution of the PIN-FORMED (PIN) gene family in Glycine max
Genome-wide identification and evolution of the PIN-FORMED (PIN) gene family in Glycine max
复制标题
大豆 PIN-FORMED (PIN) 基因家族的全基因组鉴定和进化
DOI：
10.1139/gen-2016-0141
复制
发表时间：
2017
期刊：
影响因子：
3.1
通讯作者：
Haichao Wei
中科院分区：
文献类型：
--
作者：
Liu Yuan;Haichao Wei
大豆（Glycine max）是重要的农作物之一。野生大豆和栽培大豆品种在植株形态、种子大小、种皮发育等方面存在显著差异，这些差异可能与生长素生物学有关。PIN基因家族编码细胞间生长素转运的重要转运蛋白，但对大豆PIN基因（GmPIN基因）的研究很少，尤其是野生大豆和栽培大豆的进化和差异研究较少。在本研究中，我们从最新更新的G. max基因组数据库;与之前的数据库相比，改变了6个GmPIN蛋白序列。根据植物基因组复制数据库，已发现18个GmPIN基因参与片段复制。在第二次大豆基因组复制后，出现了三对GmPIN基因，第一次基因组复制后出现了六对GmPIN基因。重复的GmPIN基因保持相似的表达模式。所有重复的GmPIN基因都经历了纯化选择（Ka/Ks < 1）以防止非同义突变的积累，从而保持更相似。此外，我们还对大豆PIN基因的人工选择进行了研究。通过比较17个野生大豆和14个栽培大豆品种的基因组序列，鉴定出5个人工筛选的GmPIN基因。我们的研究为深入了解GmPIN基因提供了有用和全面的基础信息。
Soybean (Glycine max) is one of the most important crop plants. Wild and cultivated soybean varieties have significant differences worth further investigation, such as plant morphology, seed size, and seed coat development; these characters may be related to auxin biology. The PIN gene family encodes essential transport proteins in cell-to-cell auxin transport, but little research on soybean PIN genes (GmPIN genes) has been done, especially with respect to the evolution and differences between wild and cultivated soybean. In this study, we retrieved 23 GmPIN genes from the latest updated G. max genome database; six GmPIN protein sequences were changed compared with the previous database. Based on the Plant Genome Duplication Database, 18 GmPIN genes have been involved in segment duplication. Three pairs of GmPIN genes arose after the second soybean genome duplication, and six occurred after the first genome duplication. The duplicated GmPIN genes retained similar expression patterns. All the duplicated GmPIN genes experienced purifying selection (Ka/Ks < 1) to prevent accumulation of non-synonymous mutations and thus remained more similar. In addition, we also focused on the artificial selection of the soybean PIN genes. Five artificially selected GmPIN genes were identified by comparing the genome sequence of 17 wild and 14 cultivated soybean varieties. Our research provides useful and comprehensive basic information for understanding GmPIN genes.