CELLULAR AND NUCLEAR VOLUME OF HUMAN-CELLS DURING THE CELL-CYCLE
CELLULAR AND NUCLEAR VOLUME OF HUMAN-CELLS DURING THE CELL-CYCLE
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DOI：
10.1007/bf01324188
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发表时间：
1981-01-01
影响因子：
1.7
通讯作者：
FEINENDEGEN, LE
中科院分区：
文献类型：
--
作者：
FIDORRA, J;MIELKE, T;FEINENDEGEN, LE
用库尔特计数器和扫描显微分光光度法研究了培养的人细胞在同步化生长后不同时间的全细胞体积和细胞核体积。细胞体积的增加与细胞周期中的线性或指数增长是相容的。细胞核体积的增大与DNA合成的开始无关。细胞核体积在DNA合成前6h就已经开始增大。显然，核体积的增长可以分两个阶段进行。讨论了这一结果与辐射感度的关系。
The volumes of whole cells and nuclei of cultured human cells were studied at different times after synchronization of growth using the Coulter counter and scanning microphotometry. The increase in cell volume is compatible with both linear or exponential growth during the cell cycle. The growth of the nuclear volume is not correlated with the beginning of the DNA synthesis. The nuclear volume starts to increase already 6 h prior to DNA synthesis. Apparently, the nuclear volume growth could proceed in 2 stages. The relation of this result to radiation sensitivity is discussed.