A fate map of chick otic cup closure reveals lineage boundaries in the dorsal otocyst
A fate map of chick otic cup closure reveals lineage boundaries in the dorsal otocyst
复制标题
DOI：
10.1006/dbio.2000.9914
复制
发表时间：
2000-11-15
影响因子：
2.7
通讯作者：
Fekete, DM
中科院分区：
文献类型：
--
作者：
Brigande, JV;Iten, LE;Fekete, DM
脊椎动物内耳结构复杂，由充满液体的小管和有助于听觉和平衡功能的感觉器官组成。内耳的上皮部分源自耳基板，耳基板在闭合形成耳泡之前加深形成杯状。我们对耳杯的边缘进行了命运定位，以监测与耳囊形成相关的细胞运动，并帮助解释早期耳场基因表达模式的变化。12个网站周围的边缘，定义为一个时钟面的位置，有针对性的离子电渗注射荧光，亲脂性染料。在注射后24和48小时对标记的细胞进行成像。数据显示，耳杯的整个背缘成为内淋巴管（艾德），而后腹缘成为外侧耳囊壁。在背极附近确定了两个交叉的谱系限制边界：一个将艾德分为前半部分和后半部分，另一个定义了其侧缘。我们假设跨隔室边界的信号传导可能在导管规格中起关键作用。该模型的背景下，讨论了小鼠突变体，是有缺陷的后脑发育和艾德生长。(C)北京大学出版社.
The vertebrate inner ear is structurally complex, consisting of fluid-filled tubules and sensory organs that subserve the functions of hearing and balance. The epithelial parts of the inner ear are derived from the otic placode, which deepens to form a cup before closing to form the otic vesicle. We fate-mapped the rim of the otic cup to monitor the cellular movements associated with otocyst formation and to aid in interpreting the changing gene expression patterns of the early otic field. Twelve sites around the rim, defined as positions of a clock face, were targeted by iontophoretic injection of fluorescent, lipophilic dye. Labeled cells were imaged 24 and 48 h after injection. The data show that the entire dorsal rim of the otic cup becomes the endolymphatic duct (ED), while the posteroventral rim becomes the lateral otocyst wall. Two intersecting boundaries of lineage restriction were identified near the dorsal pole: one bisecting the ED into anterior and posterior halves and the other defining its lateral edge. We hypothesize that signaling across compartment boundaries may play a critical role in duct specification. This model is discussed in the context of mouse mutants that are defective in both hindbrain development and ED outgrowth. (C) 2000 Academic Press.