Distribution of Flunixin Residues in Muscles of Dairy Cattle Dosed with Lipopolysaccharide or Saline and Treated with Flunixin by Intravenous or Intramuscular Injection.
Distribution of Flunixin Residues in Muscles of Dairy Cattle Dosed with Lipopolysaccharide or Saline and Treated with Flunixin by Intravenous or Intramuscular Injection.
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DOI：
10.1021/acs.jafc.6b04792
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发表时间：
2016-12
影响因子：
6.1
通讯作者：
W. Shelver;Marilyn J. Schneider;David J. Smith
中科院分区：
文献类型：
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作者：
W. Shelver;Marilyn J. Schneider;David J. Smith
20头奶牛接受2.2 mg/kg bw氟尼辛葡甲胺，通过静脉（IV）或肌内（IM）途径每日一次给药，连续3天，静脉注射生理盐水（NS）或脂多糖（LPS），提供每组5只动物的平衡设计。在4天停药期后处死奶牛，采集13种肌肉类型并通过LC-MS/MS测定氟尼辛。消除样本离群值后，给药途径（IV或IM）、给药（NS或LPS）和组织类型的主要影响显著（P 0.05）。肌内（非标记）氟尼辛给药在肌肉中产生的氟尼辛残留量高于IV（标记）给药（P < 0.05），而LPS导致氟尼辛水平较低。组织水平之间的差异表明，有必要指定用于监测药物水平的组织，以保护消费者。
Twenty dairy cows received flunixin meglumine at 2.2 mg/kg bw, administered once daily by either the intravenous (IV) or intramuscular (IM) route for three consecutive days with either intravenous normal saline (NS) or lipopolysaccharide (LPS) providing a balanced design with five animals per group. Cows were sacrificed after a 4 day withdrawal period, and 13 muscle types were collected and assayed for flunixin by LC-MS/MS. After elimination of sample outliers, the main effects of route of administration (IV or IM), treatment (NS or LPS), and tissue type significantly (P 0.05). Intramuscular (nonlabel) flunixin administration produced greater (P < 0.05) flunixin residues in muscle than the IV (label) administration, whereas LPS resulted in lower flunixin levels. Differences among the tissue levels indicate it is necessary to specify the tissue to be used for any monitoring of drug levels for consumer protection.