Bipolar cells of the mouse retina: a gene gun, morphological study.
Bipolar cells of the mouse retina: a gene gun, morphological study.
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小鼠视网膜双极细胞：基因枪，形态学研究。
DOI：
10.1002/cne.20207
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发表时间：
2004
期刊：
影响因子：
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通讯作者：
Strettoi,Enrica
中科院分区：
文献类型：
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作者：
Pignatelli,Vincenzo;Strettoi,Enrica
关于我们对小鼠视网膜组织的理解的关键要素之一是各种类型双极细胞的完整分类。通过本研究，我们试图为这一重要问题做出贡献。不幸的是，大多数特异性染色其他哺乳动物视网膜双极细胞的抗体几乎不适用于小鼠视网膜。我们成功地克服了这一限制，通过使用一个相对新颖的技术的基础上基因枪转移荧光染料的细胞。因此，我们能够染色相当数量的双极细胞，可以根据形态学和比较标准进行表征。我们还进行了一个完整的形态测定分析的一个子集的双极细胞染色的抗神经激肽-3受体抗体。我们发现了九种类型的锥双极细胞和一种类型的杆双极细胞;这些数据与先前对其他哺乳动物（如兔、大鼠和猴）视网膜的研究结果以及最近基于小鼠视网膜的研究结果一致（Ghosh et al. [2004] J Comp Neurol 469：70-82）。我们的研究结果也证实了哺乳动物视网膜之间存在着共同的结构相似性。各种类型的视锥双极细胞的确切功能作用以及它们对给定视觉刺激的感知的具体贡献仍有待阐明。最有可能的是，每种双极细胞类型都构成了一个专门的通道，用于计算视觉刺激的选定成分。还可以假定涉及各种类型的双极细胞的协调活性的更复杂的信号编码，如已经针对神经节细胞所示的（Meister [1996] Proc Natl Acad Sci USA 93：609-614）。J. Comp.神经元© 2004 Wiley-Liss，Inc.
One of the key elements concerning our understanding of the organization of the mouse retina is the complete classification of the various types of bipolar cells. With the present study, we tried to contribute to this important issue. Unfortunately, most of the antibodies that stain specifically bipolar cells in the retina of other mammals hardly work for the retina of the mouse. We succeeded in overcoming this limitation by using a relatively novel technique based on the gene gun transfer of fluorescent dyes to cells. Hence, we were able to stain a considerable number of bipolar cells that could be characterized according to morphological and comparative criteria. We also performed a complete morphometric analysis of a subset of bipolar cells stained by anti–neurokinin-3 receptor antibodies. We found nine types of cone bipolar cells and one type of rod bipolar cell; these data are consistent with the findings of previous studies on the retinas of other mammals, such as rabbits, rats, and monkeys and with a recent study based on the mouse retina (Ghosh et al.[2004] J Comp Neurol 469: 70–82). Our results also confirm the existence of a common structural similarity among mammalian retinas. It remains to be elucidated what is exactly the functional role of the various types of cone bipolar cells and what is the specific contribution they provide to the perception of a given visual stimulus. Most probably, each bipolar cell type constitutes a specialized channel for the computation of a selected component of the visual stimulus. More complex signal coding, involving the coordinated activity of various types of bipolar cells, could also be postulated, as it has been shown for ganglion cells (Meister [1996] Proc Natl Acad Sci USA 93: 609–614). J. Comp. Neurol. 476: 254–266, 2004.© 2004 Wiley-Liss, Inc.