Detection of duffy antigen in the plasma membranes and caveolae of vascular endothelial and epithelial cells of nonerythroid organs
Detection of duffy antigen in the plasma membranes and caveolae of vascular endothelial and epithelial cells of nonerythroid organs
复制标题
DOI：
10.1182/blood.v89.2.701
复制
发表时间：
1997-01-15
期刊：
影响因子：
20.3
通讯作者：
Pogo, AO
中科院分区：
文献类型：
--
作者：
Chaudhuri, A;Nielsen, S;Pogo, AO
Duffy血型蛋白（gp-Fy）的非红系表达仅限于某些细胞类型。肾的免疫细胞化学研究显示gp-Fy存在于肾小球内皮、小管周围毛细血管、直血管和集管的主要细胞（上皮细胞）中。大静脉内皮细胞和肺泡上皮细胞（i型）也产生Gp-Fy。在甲状腺中，只有毛细血管内皮细胞产生gp-Fy。脾毛细血管内皮细胞、高内皮小静脉和窦状窦产生丰富的gp-Fy。超微结构研究显示，包括小泡在内的根尖和基外侧质膜域存在gp-Fy。免疫印迹分析显示，gp-Fy在非红细胞中的含量明显低于在红细胞中的含量。此外，所分析的duffy阴性个体的非红细胞器官并没有产生更多的gp-Fy来弥补红细胞中这种蛋白质的缺乏。duffy阳性个体肾脏mRNA的核苷酸序列和大小与骨髓相同。因此，假定非红系Duffy蛋白与骨髓中的Duffy蛋白是同一基因的产物。非红系和红系gp-Fy具有相同的抗原结构域，这一事实加强了这一概念。(C) 1997年由美国血液病学会出版。
The nonerythroid expression of the Duffy blood group protein (gp-Fy) was confined to certain cell types. Immunocytochemistry studies of the kidney showed gp-Fy in the endothelium of glomeruli, peritubular capillaries, vasa recta, and the principal cells (epithelial) of collecting ducts. Gp-Fy was also produced in the endothelial cells of large venules and epithelial cells (type-I) of pulmonary alveoli. In the thyroid, only the endothelial cells of capillaries produced gp-Fy. In the spleen, the endothelial cells of capillaries, high endothelial venule, and sinusoids produced abundant gp-Fy. Ultrastructural studies showed that apical and basolateral plasma membrane domains, including caveolae, had gp-Fy. Immunoblot analysis showed substantially less gp-Fy in nonerythroid cells than in erythrocytes. Moreover, the analyzed nonerythroid organs of Duffy-negative individuals did not produce more gp-Fy to compensate for the lack of this protein in their erythrocytes. The nucleotide sequence and the size of kidney mRNA from a Duffy-positive individual were the same as that of bone marrow. It is assumed, therefore, that nonerythroid Duffy protein is the product of the same gene as that of bone marrow. This notion is reinforced by the fact that nonerythroid and erythroid gp-Fy have the same antigenic domains. (C) 1997 by The American Society of Hematology.