Profiling of the secreted proteins during 3T3-L1 adipocyte differentiation leads to the identification of novel adipokines
Profiling of the secreted proteins during 3T3-L1 adipocyte differentiation leads to the identification of novel adipokines
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DOI：
10.1007/s00018-004-4256-z
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发表时间：
2004-09-01
影响因子：
8
通讯作者：
Renes, J
中科院分区：
文献类型：
--
作者：
Wang, P;Mariman, E;Renes, J
脂肪组织是一种内分泌器官，能分泌多种脂肪因子，对脂肪组织和全身代谢起调节作用。我们使用二维凝胶电泳结合质谱分析（前）脂肪细胞分泌的蛋白质。筛选了脂肪细胞分化过程中3 T3-L1细胞的培养基，并鉴定了41种响应于20 ℃处理和/或布雷菲德菌素A处理的分泌阻断的蛋白质。首次报道了抑制素、应激蛋白70和粘附调节分子1为分泌蛋白。此外，前胶原蛋白C-蛋白酶增强蛋白、半乳糖凝集素-1、亲环素A和C以及SF 20/IL-25是新鉴定的脂肪细胞分泌因子。分泌概况表明一个动态的环境，包括积极重塑细胞外基质和参与生长调节的几个因素。
Adipose tissue is an endocrine organ capable of secreting a number of adipokines with a role in the regulation of adipose tissue and whole-body metabolism. We used two-dimensional gel electrophoresis combined with mass spectrometry to profile the secreted proteins from (pre)adipocytes. The culture medium of 3T3-L1 cells during adipocyte differentiation was screened, and 41 proteins that responded to blocking of secretion by 20degreesC treatment and/or brefeldin A treatment were identified. Prohibitin, stress-70 protein, and adhesion-regulating molecule 1 are reported for the first time as secreted proteins. In addition, procollagen C-proteinase enhancer protein, galectin-1, cyclophilin A and C, and SF20/IL-25 are newly identified as adipocyte secreted factors. Secretion profiles indicated a dynamic environment including an actively remodeling extracellular matrix and several factors involved in growth regulation.