Quantitative and real-time detection of secretion of chemical messengers from individual platelets
Quantitative and real-time detection of secretion of chemical messengers from individual platelets
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DOI：
10.1021/bi800792m
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发表时间：
2008-07-08
期刊：
影响因子：
2.9
通讯作者：
Haynes, Christy L.
中科院分区：
文献类型：
--
作者：
Ge, Shencheng;Wittenberg, Nathan J.;Haynes, Christy L.
本研究利用碳纤维微电化学法测定洗涤后的兔血小板分泌5-羟色胺和组胺的个体胞吐事件。定量表征了5-羟色胺的定量释放，其三角洲颗粒5-羟色胺浓度为0.6M，分泌时间为7ms。此外，细胞外渗透压影响量子大小，在低渗条件下导致量子大小增加，这可能是由于胞浆中的5-羟色胺流入三角洲颗粒的晕区。
Carbon-fiber microelectrochemical methods were utilized in this study to measure individual exocytotic events of secretion of serotonin and histamine from washed rabbit platelets. The quantal release of serotonin was quantitatively characterized with a delta-granule serotonin concentration of 0.6 M and secretion time course of 7 ms. Additionally, extracellular osmolarity influences quantal size, causing quantal size increases under hypotonic conditions, presumably due to the influx of cytosolic serotonin into the halo region of the delta-granules.