Calcium ionophore induced proteolysis and cataract: Inhibition by cell permeable calpain antagonists
Calcium ionophore induced proteolysis and cataract: Inhibition by cell permeable calpain antagonists
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DOI：
10.1006/bbrc.1996.0159
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发表时间：
1996-01-26
影响因子：
3.1
通讯作者：
Duncan, G
中科院分区：
文献类型：
--
作者：
Sanderson, J;Marcantonio, JM;Duncan, G
两个二肽醛细胞渗透钙蛋白酶抑制剂，cBz-Val-Phe和calpeptin，已被评估其能力，以防止细胞骨架蛋白水解和损失的透明度，在整个大鼠晶状体。离子霉素在含1 mM钙的人工房水中诱导钙超载，导致透镜混浊和细胞骨架蛋白（包括血影蛋白、filensin和波形蛋白）降解。在无钙条件下，在离子霉素中孵育未导致此类变化。在钙超载透镜抑制剂提供了一些保护，防止细胞骨架蛋白降解和透明度的损失。这些实验表明钙蛋白酶在高钙晶状体的皮质混浊中具有作用，并且细胞穿透钙蛋白酶抑制剂确实进入透镜细胞并减少蛋白水解和混浊。(C)出版社：Academic Press，Inc.
Two dipeptide aldehyde cell permeable calpain inhibitors, cBz-Val-Phe and calpeptin, have been assessed for their ability to prevent cytoskeletal proteolysis and loss of transparency in whole rat lenses. Calcium overload, induced by ionomycin in artificial aqueous humor with lmM calcium, resulted in lens opacification and degradation of cytoskeletal proteins including spectrin, filensin, and vimentin. No such changes resulted from incubation in ionomycin in the absence of calcium. In calcium overload lenses both inhibitors gave some protection against cytoskeletal protein degradation and loss of transparency. These experiments indicate that calpain has a role in cortical opacification in high calcium lenses and that cell penetrating calpain inhibitors do indeed enter lens cells and reduce both proteolysis and opacification. (C) 1996 Academic Press, Inc.